Developmental trial of a diagnosis kit on the disease activity of alveolar bone resofptibn in periodontitis patients.

牙周炎患者牙槽骨吸收疾病活动性诊断试剂盒的开发试验。

• 批准号: 11557168
• 负责人: NAGATA Toshihiko
• 金额: $ 5.44万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11557168/
• 关键词: gingival crevicular fluid; osteopontin; osteocalcin; calprotectin; P1CP; bone resorption; periodontal disease; 骨吸收; 歯周炎

To determine the clinical marker reflecting alveolar bone resorption in periodontal disease, osteocalcin (OCN), osteopontin (OPN), carboxy terminal propeptide of type I procollagen (P1CP), and calprotectin (CPT) were biochemically quantified using gingival crevicular fluid (GCF) from periodontitis patients. These markers appeared in GCF from periodontitis patients, whereas they were also detected a little in GCF from healthy subjects. The levels of P1CP and CPT strongly correlated with clinical inflammatory indicators such as gingival index and probing depth. Moreover, P1CP and CPT correlated with bleeding on probing (BOP) which is associated with the disease activity. OPN and OCN weakly correlated with the inflammatory markes. P1CP showed high level in GCF from bone resorptive area. Taken together, it is suggested that CPT may be a potent marker reflecting inflammatory activity and P1CP may be a potent marker reflecting bone resorption in periodontal disease.

为了确定反映牙周病牙槽骨吸收的临床标志物，采用牙周炎患者龈沟液（GCF）对骨钙素（OCN）、骨桥蛋白（OPN）、I型前胶原羧基末端前肽（P1CP）和钙保护蛋白（CPT）进行生化定量分析。这些标志物出现在牙周炎患者的GCF中，而在健康受试者的GCF中也很少检测到。P1CP、CPT水平与牙龈指数、探诊深度等临床炎症指标密切相关。此外，P1CP和CPT与探查出血（BOP）相关，BOP与疾病活动性相关。OPN和OCN与炎症灶呈弱相关。骨吸收区GCF中P1CP含量较高。综上所述，CPT可能是反映炎症活动的有效标志物，而P1CP可能是反映牙周病骨吸收的有效标志物。

项目成果

永田俊彦: "「骨代謝以上」(330-334)「先端医療シリーズ・歯周病・新しい治療を求めて」"岡田宏,石川烈,村山洋二,監修:先端医療技術研究所,東京. 536 (2000)

Toshihiko Nagata：“不仅仅是骨代谢”（330-334）“高级医学系列：牙周病：寻找新的治疗方法”Hiroshi Okada、Retsu Ishikawa、Yoji Murayama，由先进医疗技术研究所监督，东京 536（2000 年）。 ）

Nagata,T.: "Disorders of bone metabolism"SENTAN IRYOU SIRIESE SIKAIGAKU. 330-334 (2000)

Nagata,T.：“骨代谢紊乱”SENTAN IRYOU SIRIESE SIKAIGAKU。

中村輝夫: "歯周炎患者の歯肉溝滲出液中に含まれるI型プロコラーゲンペプチドおよびオステオカルツンの定量分析"日本歯周病学会会誌. 44・(1)(印刷中). (2002)

Teruo Nakamura：“牙周炎患者龈沟液中所含的 I 型前胶原肽和骨软骨蛋白的定量分析”日本牙周病学会杂志 44·(1)（出版中）。

Teruo Nakamura: "The association of calprotectin level in gingival crevicular fluid with gingival index and the activities of collagenase and AST in periodontitis"Journal of Periodontology. 71. 361-367 (2000)

Teruo Nakamura：“牙周炎中龈沟液中钙卫蛋白水平与牙龈指数以及胶原酶和 AST 活性的关系”《牙周病学杂志》。

Nakamura,T., Kido,J., Kido,R., Ohishi,K., Yamauchi,N., Kataoka,M., Nagata,T.: "The association of calprotectin level in gingival crevicular fluid with gingival index and the activities of collagenase and aspartate aminotransferase in adult periodontitis p

Nakamura,T.、Kido,J.、Kido,R.、Ohishi,K.、Yamauchi,N.、Kataoka,M.、Nagata,T.：“龈沟液中钙卫蛋白水平与牙龈指数和牙龈指数的关系