Real-time observation of proton-pumping of membrane protein complexes using a novel high-sensitivity IR spectrometer

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This project aims to establish time-resolved IR spectroscopic techniques for observing reactions of membrane protein complexes under physiological conditions in real time. High-sensitivity IR spectrometer was constructed using a femtosecond IR laser as the light source. On the other hand, a flow-flash measurement system was developed to control reactions, which are triggered by the photolysis of caged compounds. Micro-channel flow cell was used to obtain a spectrum with minimal sample consumption. The flow-flash system was installed into time-resolved UV-visible absorption spectrometer and tested on NO reductase. We successfully observed the enzymatic reaction of this membrane protein with microsecond time resolution. Flow-flash time-resolved IR spectroscopy is ongoing.