Elucidation of genetic diversity and genetic management in captive breeding of endangered amphibian species endemic to Ryukyu Islands

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Genetic diversity of represents the evolutionary history of species and the essential evolutionary potential to respond to changing environments especially for endangered species. Microsatellites are currently the most practical and informative molecular maker for measuring genetic variation within populations. In this study, we developed microsatellite markers for nine endangered amphibian species endemic to Ryukyu Islands in Japan. By using these markers, fine scale population structures and genetic diversities of these species were revealed. These results have contributed to effective conservation and enabled genetic management of ex-situ conservation activities of these endangered amphibians.