Raman tag imaging for viasualization of small molecules in live cells

炔基标签拉曼成像是一种利用炔基标签对小分子进行活细胞成像的方法。以EDU为标准，研究了不同烷烃的结构-拉曼位移/强度关系。在所得结果的基础上，实现了两个炔标记分子EDU和炔标记辅酶Q类似物(AltQ)的同时成像，并半定量地估计了辅酶Q类似物的细胞浓度。最后，成功地实现了质子化和去质子化的对三氟甲氧基苯肼(FCCP)的同时成像。

Alkyne-tag Raman imaging was proposed as an approach for live cell imaging of small molecules by using an alkyne-tag. Structure-Raman shift/intensity relationships of various alkynes was examined by use of EdU as standard. On the basis of obtained results, simultaneous imaging of two alkyne-tagged molecules, EdU and alkyne-tagged CoQ analogue (AltQ), and semiquantitative estimation of cellular concentration of CoQ analogues have been achieved. Finally, simultaneous imaging of protonated and deprotonated carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP) was successfully demonstrated.