The role of adhesion molecules in morphogenesis of the olfactory epithelium during development and regeneration

在嗅上皮（OE）的顶端表面，嗅觉细胞和支持细胞呈典型的马赛克状排列。然而，细胞模式是如何建立的尚不清楚。我们阐明了粘附分子在OE细胞模式中的作用。在OE的发育和再生过程中，嗅觉细胞和支持细胞发生了重排，并以不同的模式表达了各种类型的粘附分子，如连接素和钙粘蛋白。在nectin-KO小鼠的OE中，细胞模式受到干扰。为了验证粘附分子在OE细胞模式形成中的作用，我们进行了混合细胞培养实验。将粘附分子的选择性基因转移到没有粘附分子的细胞系中。这些细胞在培养中排列成独特的马赛克图案。这些结果表明，粘附分子有助于OE的细胞模式形成。

In the apical surface of the olfactory epithelium (OE), olfactory cells and supporting cells are arranged in a characteristic mosaic pattern. However, how the cellular pattern is established is still unclear. We clarified the role of adhesion molecules in the cellular patterning of the OE. During development and regeneration of the OE, olfactory cells and supporting cells became rearranged, which expressed various types of adhesion molecules, nectins and cadherins, in various patterns. In the OE of nectin-KO mice, the cellular pattern was disturbed. To validate the role of adhesion molecules in the cellular pattern formation of the OE, we performed mixed cell culture experiments. Optional genes of adhesion molecules were transferred to cell lines without each adhesion molecule. These cells were arranged into characteristic mosaic pattern in culture. These results suggest that adhesion molecules contribute to the cellular pattern formation of the OE.