The comprehensive profile analysis for regional metastatic potential of head and neck squamous cell carcinoma in vivo

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To identify the mechanism of regional metastasis of head and neck squamous cell carcinoma (HNSCC), we characterized regional metastatic potential in vivo using an orthotopic mouse model of HNSCC first. For further study, 15 HNSCC cell lines, establishing more than 80 % of tongue tumor formation in the model, were selected and whole gene microarray was performed. According to the result of principal component analysis based on all the probe sets utilized in our microarray analysis, 4 cells were selected for further analysis. Genes with an absolute fold change value > 1.5 were selected for further analysis.The upstream and key nodes analysis with ExPlain® was then used to investigate upstream key molecules involved in the mechanisms of regional metastasis of HNSCC. The results showed molecules in AP-1 family with higher score suggesting that those molecules could be considered as key factors regulating the pathways related with regional metastasis of HNSCC.