The comprehensive profile analysis for regional metastatic potential of head and neck squamous cell carcinoma in vivo

头颈部鳞状细胞癌体内局部转移潜能综合分析

• 批准号: 24791797
• 负责人: SANO Daisuke
• 金额: $ 2.75万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791797/
• 关键词: 頭頸部癌; 頸部リンパ節; 同所性頭頸部癌モデル; 頸部リンパ節転移

To identify the mechanism of regional metastasis of head and neck squamous cell carcinoma (HNSCC), we characterized regional metastatic potential in vivo using an orthotopic mouse model of HNSCC first. For further study, 15 HNSCC cell lines, establishing more than 80 % of tongue tumor formation in the model, were selected and whole gene microarray was performed. According to the result of principal component analysis based on all the probe sets utilized in our microarray analysis, 4 cells were selected for further analysis. Genes with an absolute fold change value > 1.5 were selected for further analysis.The upstream and key nodes analysis with ExPlain® was then used to investigate upstream key molecules involved in the mechanisms of regional metastasis of HNSCC. The results showed molecules in AP-1 family with higher score suggesting that those molecules could be considered as key factors regulating the pathways related with regional metastasis of HNSCC.

为了明确头颈部鳞状细胞癌(HNSCC)的区域转移机制，我们首先利用HNSCC原位小鼠模型在体内研究了HNSCC的区域转移潜能。为了进一步研究，选择了15个HNSCC细胞系，在该模型中建立了80%以上的舌癌成瘤率，并进行了全基因芯片。根据芯片分析中使用的所有探针集的主成分分析结果，选择4个细胞进行进一步分析。选择具有绝对折叠变化值的基因&gt；1.5进行进一步分析，然后使用EXPLAIN®对上游和关键节点进行分析，以研究参与HNSCC区域转移机制的上游关键分子。结果显示AP-1家族中的分子得分较高，提示这些分子可能是调控HNSCC区域转移相关通路的关键因素。

项目成果

The regional metastatic potential of an orthotopic mouse model of head and neck squamous cell carcinoma

头颈鳞状细胞癌原位小鼠模型的区域转移潜力

• 发表时间: 2012
• 作者: Sano D;Takahashi H
• 通讯作者: Takahashi H

The profile of regional and distant metastatic potential of head and neck squamous cell carcinoma in vivo

头颈鳞状细胞癌体内局部和远处转移潜能概况

• 发表时间: 2013
• 作者: Sano D;Hyakusoku H;Takahashi H;Oridate N
• 通讯作者: Oridate N

頭頸部癌研究における経尾静脈移植による頭頸部癌肺転移モデルの有用性についての検討

尾静脉移植头颈癌肺转移模型在头颈癌研究中的有效性检验

• 发表时间: 2013
• 作者: 百束 紘;高橋秀聡;堀井千裕;西村剛志;高橋優宏;田口享秀;佐野大佑
• 通讯作者: 佐野大佑

The applicability of murine orthotopic and experimental metastasis models for identifying the mechanisms of regional and distant metastasis of head and neck squamous cell carcinoma

小鼠原位和实验转移模型在鉴定头颈鳞状细胞癌区域和远处转移机制中的适用性

• 发表时间: 2012
• 作者: Sano D;Hyakusoku H;Takahashi H;Nishimura G;Takahashi M;Taguchi T;Matusda H;Myers JN
• 通讯作者: Myers JN

同所性頭頸部癌モデルを用いた頭頸部癌頸部リンパ節転移の機序の解明

使用原位头颈癌模型阐明头颈癌颈淋巴结转移的机制

• 发表时间: 2013
• 期刊: 横浜医学
• 作者: Bando H;Ahmed ME;El-Deen EM;Eman AM;El-Adawy AA;Kotby MN;and Hisa Y.;Sen Matayoshi;又吉宣;佐野大佑
• 通讯作者: 佐野大佑