The comprehensive profile analysis for regional metastatic potential of head and neck squamous cell carcinoma in vivo

为了明确头颈部鳞状细胞癌（HNSCC）区域转移的机制，我们首先使用原位HNSCC小鼠模型表征了体内区域转移潜力。为了进一步研究，选择了在模型中建立超过80%的舌肿瘤形成的15个HNSCC细胞系，并进行全基因芯片。根据主成分分析的结果，在我们的微阵列分析中使用的所有探针集的基础上，选择4个细胞进行进一步分析。选择绝对倍数变化值> 1.5的基因进行进一步分析，然后使用ExPlain®进行上游和关键节点分析，以研究参与HNSCC区域转移机制的上游关键分子。结果显示AP-1家族分子在HNSCC区域转移中的表达较高，提示AP-1家族分子可能是HNSCC区域转移相关通路的关键调控因子。

To identify the mechanism of regional metastasis of head and neck squamous cell carcinoma (HNSCC), we characterized regional metastatic potential in vivo using an orthotopic mouse model of HNSCC first. For further study, 15 HNSCC cell lines, establishing more than 80 % of tongue tumor formation in the model, were selected and whole gene microarray was performed. According to the result of principal component analysis based on all the probe sets utilized in our microarray analysis, 4 cells were selected for further analysis. Genes with an absolute fold change value > 1.5 were selected for further analysis.The upstream and key nodes analysis with ExPlain® was then used to investigate upstream key molecules involved in the mechanisms of regional metastasis of HNSCC. The results showed molecules in AP-1 family with higher score suggesting that those molecules could be considered as key factors regulating the pathways related with regional metastasis of HNSCC.