The application of objective measurement of tinnitus using BDNF to clarify the pathomechanism of tinnitus

应用BDNF客观测量耳鸣阐明耳鸣发病机制

• 批准号: 24791805
• 负责人: SAITO Tomoko
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791805/
• 关键词: 耳鳴; BDNF; autophagy; バイオマーカー; 内耳感覚細胞

We try to establish the tinnitus model with inner ear cultured cell line HEI-OC1. At the beginning tunicamycin as endoplasmic reticulum stress was applied to HEI-OC1. After that inner ear tinnitus model was designed as the condition (concentration of 100 microgram/ml, and processing time of 0-48 hours), in which the cell validity was significantly different between the control and tunicamycin (+) condition. In this condition, the time dependant gradual reduction of appearance of BDNF was confirmed by western blot. In addition with this unique condition Atoh1,inner ear marker was also time dependently reduced.

本研究尝试用内耳培养细胞系HEI-OC 1建立耳鸣模型。在开始时，衣霉素作为内质网应激被施加到HEI-OC 1。在此基础上设计了内耳耳鸣模型的条件（浓度为100 μ g/ml，处理时间为0-48 h），其中对照组和衣霉素（+）组的细胞有效性有显著性差异。在这种情况下，通过蛋白质印迹证实BDNF的出现的时间依赖性逐渐减少。除了这种独特的条件Atoh 1，内耳标志物也随时间而减少。

项目成果

HOW ANXIETY AFFECT ON ANTERO-POSTERIOR POSTURAL STABILITY IN PATIENTS WITH DIZZINESS ?

焦虑如何影响头晕患者的前后姿势稳定性？

• 发表时间: 2013
• 作者: Fumiyuki GOTO;Tomoko TSUTSUMI-SAITO;Kaoru OGAWA
• 通讯作者: Kaoru OGAWA

The anxiety affects the antero-posterior postural stability in patients with dizziness

焦虑对头晕患者前后位姿势稳定性的影响

• 发表时间: 2012
• 作者: Fumiyuki Goto;Tomoko Tsutsumi;Keisuke Kushiro
• 通讯作者: Keisuke Kushiro

Hidden depression in otolaryngology patients with medically unexplained symptoms

• DOI: 10.1016/j.genhosppsych.2011.09.014
• 发表时间: 2012-03-01
• 期刊: GENERAL HOSPITAL PSYCHIATRY
• 影响因子: 7
• 作者: Goto, Fumiyuki;Tsutsumi, Tomoko;Mimura, Masaru
• 通讯作者: Mimura, Masaru

Objective evaluation of neck muscle tension and static balance in patients with chronic dizziness

慢性头晕患者颈部肌张力及静态平衡的客观评价

• 发表时间: 2012
• 期刊: Acta Otolaryngol
• 作者: Asama Y;Goto F;Tsutsumi T
• 通讯作者: Tsutsumi T

THE BIOMARKER OF TINNTUS

Tinntus 的生物标志物

• 发表时间: 2012
• 作者: Goto F.;Saruta J.;Kanzaki S.;To M.;Tsutsumi T.;Tsukinoki K.;Ogawa K.
• 通讯作者: Ogawa K.