The application of objective measurement of tinnitus using BDNF to clarify the pathomechanism of tinnitus

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We try to establish the tinnitus model with inner ear cultured cell line HEI-OC1. At the beginning tunicamycin as endoplasmic reticulum stress was applied to HEI-OC1. After that inner ear tinnitus model was designed as the condition (concentration of 100 microgram/ml, and processing time of 0-48 hours), in which the cell validity was significantly different between the control and tunicamycin (+) condition. In this condition, the time dependant gradual reduction of appearance of BDNF was confirmed by western blot. In addition with this unique condition Atoh1,inner ear marker was also time dependently reduced.