Electrochemical DNA detection using naphthalene diimide having ferrocene and beta-cyclodextrin in homogenous solution

电化学DNA检测技术是结合医疗保健生物芯片等相关领域进行研究的。在均相溶液中检测PCR扩增的双链DNA以检查流感类型或诺如病毒也是重要的。这可以通过我们开发的均相溶液中双链DNA的检测系统来实现。二茂铁基萘二酰亚胺负载β-环糊精（FNC）在无DNA固定电极的条件下实现了双链DNA的信号开启型电化学检测。FNC显示二茂铁和β-环糊精之间的分子内包合复合物，具有减弱的电流信号。在与双链DNA结合后，复合物崩溃，导致恢复的电流信号，氧化还原电位正移。可以在均相溶液中以高灵敏度电化学检测PCR产物。

Electrochemical DNA detection has been studying in light of health-care biochip and other related fields. It is also important to detect double stranded DNA amplified by PCR under homogeneous solution to check influenza type or norovirus. This can be achieved our developed detection system of double stranded DNA in homogenous solution. Ferrocenylnaphthalene diimide carrying beta-cyclodextrin (FNC) provides signal-on type electrochemical detection of double stranded DNA without DNA-immobilized electrode. FNC shows intramolecular inclusion complex between ferrocene and beta-cyclodextrin with diminished current signal. After bound to double stranded DNA, the complex is collapsed resulting in recovered current signal with positive shift of redox potential. One can detect PCR product electrochemically with high sensitivity under homogenous solution.