Electrochemical DNA detection using naphthalene diimide having ferrocene and beta-cyclodextrin in homogenous solution

电化学DNA检测一直是医疗保健生物芯片等相关领域的研究热点。在均相溶液中检测PCR扩增的双链DNA对流感型或诺如病毒也很重要。这可以实现我们开发的双链DNA在均相溶液中的检测系统。二茂铁基萘二亚胺携带β -环糊精（FNC），无需DNA固定电极即可实现双链DNA的信号型电化学检测。FNC显示二茂铁与-环糊精分子内包合物，电流信号减弱。在与双链DNA结合后，复合物坍塌，导致氧化还原电位正移位的电流信号恢复。在均相溶液下，电化学检测PCR产物具有较高的灵敏度。

Electrochemical DNA detection has been studying in light of health-care biochip and other related fields. It is also important to detect double stranded DNA amplified by PCR under homogeneous solution to check influenza type or norovirus. This can be achieved our developed detection system of double stranded DNA in homogenous solution. Ferrocenylnaphthalene diimide carrying beta-cyclodextrin (FNC) provides signal-on type electrochemical detection of double stranded DNA without DNA-immobilized electrode. FNC shows intramolecular inclusion complex between ferrocene and beta-cyclodextrin with diminished current signal. After bound to double stranded DNA, the complex is collapsed resulting in recovered current signal with positive shift of redox potential. One can detect PCR product electrochemically with high sensitivity under homogenous solution.