Development of an analytical system for the high sensitive detection of point mutation in oncogene

建立了基于连接酶检测反应（LDR）的硼掺杂金刚石（BDD）电极高效液相色谱电化学检测（HPLC-ECD）突变检测方法，该方法可区分低丰度突变体DNA和高丰度野生型DNA。 用该方法对LDR产生的寡核苷酸进行了分离和检测。 通过本HPLC-ECD，检测来自KRAS密码子12.2突变的LDR产物。

High-performance liquid chromatography with electrochemical detection (HPLC-ECD) using boron-doped diamond (BDD) electrode was developed to perform the mutation detection assay based on the ligase detection reaction (LDR), which can distinguish low-abundant mutant DNA from abundant wild-type DNA. The performance of the present HPLC-ECD was examined to separate and detect an oligonucleotide which was produced by LDR. By the present HPLC-ECD, the LDR product derived from KRAS codon 12.2 mutations was detected.