Anaerobic degradation pathway of hetero-aromatic compounds

研究了杂环化合物咔唑、二苯并呋喃和二苯并噻吩的厌氧生物降解。能够降解杂环化合物的细菌菌株从富集培养物中分离，其中杂环化合物和不溶性Fe（III）氧化物分别作为唯一的电子供体和受体加入。从河流和水坝沉积物和土壤中收集接种物。在富集培养物中，分别从以咔唑、二苯并呋喃和二苯并噻吩为唯一电子供体的培养基中分离出10、1和1株菌株。16S rDNA序列分析表明，其中一株咔唑降解菌属于脱硫弧菌科（Desulfovibrionaceae）。它的模式菌株不能降解咔唑，因此，它们的比较基因组分析正在进行中，以确定参与咔唑降解的基因。

Anaerobic biodegradation of heterocyclic compounds, carbazole, dibenzofuran, and dibenzothiophene were investigated. Bacterial strains capable of degrading the heterocyclic compounds were isolated from enriched culture in which the heterocyclic compounds and insoluble Fe(III) oxide were added as the sole electron donor and accepter, respectively. Inocula were collected from river and dam sediments and soils. Among the enriched cultures, 10, 1, and 1 strains were isolated from the medium in which carbazole, dibenzofuran, and dibenzothiophene were used as the sole electron donor, respectively. 16S rDNA sequencing analysis revealed one of the carbazole-degrading isolates belongs to Desulfovibrionaceae. Its type strain could not degrade carbazole and, thus, their comparative genome analysis is now underway in order to identify the gene involved in carbazole degradation.