The regulation of cytosolic chloride ion concentration which regulates autophagy system via regulation of lysosome acidity is a new target for cancer therapy

点击翻译按钮获取中文摘要

Autophagy is a catabolic process degrading cell components mediated through lysosomal machineries. Lysosome is, therefore, a key organelle in autophagy degrading various compounds. The digesting activity of lysosomal enzymes depends on intra-lysosomal acidity. we clarified roles of cytosolic chloride ion in regulation of lysosomal acidification intra-lysosomal pH and autophagy function in human cancer cell lines and mouse embryonic fibroblast cells. The lowered [Cl-]c primarily causes dysfunction of autophagy via dysfunction of lysosome induced by disturbance of intra-lysosomal acidification.