Investigation of inhibitory effects of plant extracts on toxin type food poisoning and its applications

植物提取物对毒素型食物中毒的抑制作用研究及其应用

• 批准号: 24700806
• 负责人: SHIMAMURA YUKO
• 金额: $ 1.16万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700806/
• 关键词: 黄色ブドウ球菌; ポリフェノール; エンテロトキシンA; ブドウ球菌エンテロトキシンA(SEA); ブドウ球菌エンテロトキシンA

The aims of the present study are first to investigate the binding activities of plant extracts and its components (n=136) to staphylococcal enterotoxin A (SEA), and second to examine the inhibitory effects of these extracts and components on the production and toxic activity of SEA. As a result, 26 samples inhibited the binding affinity of the anti-SEA antibody to SEA. Although highly polymerized proanthocyanidins in apple extract was bound with SEA directly, lowly polymerized proanthocyanidins inhibited the toxic activity without binding with SEA. Hydroxyl group at position 3 of galloyl group in catechin structure was responsible for binding affinity with the SEA. However methylated-catechins (methyl group at position 3 or 4 of galloyl group) showed inhibitory effect on production and toxic activity of SEA. It was suggested that these inhibitory effects were not necessarily related to the binding affinity of SEA.

本研究的目的是首先研究植物提取物及其成分（n=136）对葡萄球菌肠毒素A （SEA）的结合活性，其次研究这些提取物和成分对SEA产生和毒性活性的抑制作用。结果，26个样品抑制了抗SEA抗体对SEA的结合亲和力。虽然苹果提取物中高聚合的原花青素直接与SEA结合，但低聚合的原花青素不与SEA结合而抑制了毒活性。儿茶素结构中没食子酰基第3位的羟基负责与SEA的结合亲和力。甲基化儿茶素（没食子酰基第3位或第4位甲基）对SEA的产生和毒性活性均有抑制作用。提示这些抑制作用不一定与SEA的结合亲和力有关。

项目成果

Screening of plant extracts for inhibitory effects on the production and biological activity of bacterial toxins.

筛选植物提取物对细菌毒素产生和生物活性的抑制作用。

• 发表时间: 2012
• 作者: Natsumi Aoki;Yuko Shimamura;Takashi Tanaka;Masatsune Murata;and Shuichi Masuda
• 通讯作者: and Shuichi Masuda

食中毒菌の毒素産生および活性に対するリンゴ由来プロアントシアニジン画分の抑制効果

苹果原花青素组分对食物中毒细菌毒素产生和活性的抑制作用

• 发表时间: 2013
• 作者: 平石美季,島村裕子,新家桃香,佐藤勝浩,青野博志,青野盈瓠,篠田和代;村田容常,増田修一;杉山由華,島村裕子,柳田顕郎,増田修一
• 通讯作者: 杉山由華,島村裕子,柳田顕郎,増田修一

Solophenols and solomonin; new prenylated polyphenols isolated from propolis collected from the Solomon Islands and their antibacterial activity

所罗酚和所罗门宁；

• 发表时间: 2012
• 作者: Saori Inui;Takahiro Hosoya;Yuko Shimamura;Shuichi Masuda;Kenichi Shirafuji;Reuben Toli Moli;Shigenori Kumazawa
• 通讯作者: Shigenori Kumazawa

食中毒菌の毒素産生および活性に対するカテキン類の抑制効果

儿茶素对食物中毒细菌毒素产生和活性的抑制作用

• 发表时间: 2014
• 作者: Tsutsuura S;Shimamura Y;and Murata M;Shigenori Kumazawa他;杉山由華,島村裕子,菅敏幸,増田修一
• 通讯作者: 杉山由華,島村裕子,菅敏幸,増田修一

Screening of plant extracts for inhibitory effects on the production and biological activity of bacterial toxins

筛选植物提取物对细菌毒素产生和生物活性的抑制作用

• 发表时间: 2012
• 作者: Natsumi Aoki;Yuko Shimamura;Takashi Tanaka;Masatsune Murata;and Shuichi Masuda
• 通讯作者: and Shuichi Masuda