Investigation of inhibitory effects of plant extracts on toxin type food poisoning and its applications

本研究的目的是首先研究植物提取物及其组分(n=136)与葡萄球菌肠毒素A(SEA)的结合活性，然后检测这些提取物和组分对SEA产生和毒力的抑制作用。结果，26个样品抑制了抗SEA抗体与SEA的结合亲和力。虽然苹果提取物中高聚合度的原花青素直接与SEA结合，但低聚合度的原花青素不与SEA结合而抑制其毒性活性。儿茶素结构中没食子酸基第3位的羟基负责与SEA的结合。而甲基化儿茶素(没食子酰基3位或4位甲基)对SEA的产生和毒力有抑制作用。提示这些抑制作用与SEA的结合亲和力没有必然关系。

The aims of the present study are first to investigate the binding activities of plant extracts and its components (n=136) to staphylococcal enterotoxin A (SEA), and second to examine the inhibitory effects of these extracts and components on the production and toxic activity of SEA. As a result, 26 samples inhibited the binding affinity of the anti-SEA antibody to SEA. Although highly polymerized proanthocyanidins in apple extract was bound with SEA directly, lowly polymerized proanthocyanidins inhibited the toxic activity without binding with SEA. Hydroxyl group at position 3 of galloyl group in catechin structure was responsible for binding affinity with the SEA. However methylated-catechins (methyl group at position 3 or 4 of galloyl group) showed inhibitory effect on production and toxic activity of SEA. It was suggested that these inhibitory effects were not necessarily related to the binding affinity of SEA.