Genomic and physiological characterization of dehalorespiring bacteria in three phyla

脱卤呼吸细菌通过呼吸作用生长，使用有机卤化物作为电子受体，并将有机卤化物还原脱卤为卤代较少的化合物。我还从不同的环境样品中富集和分离了三门中在遗传学和功能上不同的脱卤呼吸细菌。其中，Geophyllsp. AY表现出前所未有的高脱氯活性，在一周内将1，2-二氯乙烷（1，2-DCA）解毒为乙烯。AY具有对高浓度1，2-DCA的高活性、仅以乙酸盐为唯一电子供体和碳源的可培养性以及在不含有机卤化物的培养物中的高活性等优点。基因组分析表明，该基因的组成型表达可能是由一个缺乏cAMP激酶转录调控因子的质粒从专性脱卤呼吸脱卤酶转移而来。

Dehalorespiring bacteria grow by respiration using organohalides as electron acceptors and reductively dehalogenate organohalides to less halogenated compounds. I have also enriched and isolated phylogenetically and functionally different dehalorespiring bacteria of the three phyla from various environmental samples. Among them, Geobacter sp. AY showed unprecedented high dechlorination activity that detoxify 1,2-dichloroethane (1,2-DCA) of 1,000 ppm to ethene within a week. Geobacter sp. AY had following advantages to be applied in bioremediation; the high activity for high concentration of 1,2-DCA, culturability using only acetate as sole electron donor and carbon source, and the high activity in the culture grown without any organohalides. The genome analysis revealed the constitutive expression was probably resulted from a plasmid lacking of cAMP kinase transcriptional regulator transferred from obligate dehalorespiring Dehalobacter.