Genomic and physiological characterization of dehalorespiring bacteria in three phyla

三个门脱盐呼吸细菌的基因组和生理学特征

• 批准号: 24710077
• 负责人: YOSHIDA Naoko
• 金额: $ 3.08万
• 依托单位: Nagoya Institute of Technology (2013)Toyohashi University of Technology (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24710077/
• 关键词: 脱ハロゲン化呼吸細菌; バイオレメディエーション; ジオバクター; デハロバクター; デハロコッコイデス; 還元的脱塩素化; Geobacter; Dehalobacter; 脱塩素化呼吸; 還元的脱ハロゲン化

Dehalorespiring bacteria grow by respiration using organohalides as electron acceptors and reductively dehalogenate organohalides to less halogenated compounds. I have also enriched and isolated phylogenetically and functionally different dehalorespiring bacteria of the three phyla from various environmental samples. Among them, Geobacter sp. AY showed unprecedented high dechlorination activity that detoxify 1,2-dichloroethane (1,2-DCA) of 1,000 ppm to ethene within a week. Geobacter sp. AY had following advantages to be applied in bioremediation; the high activity for high concentration of 1,2-DCA, culturability using only acetate as sole electron donor and carbon source, and the high activity in the culture grown without any organohalides. The genome analysis revealed the constitutive expression was probably resulted from a plasmid lacking of cAMP kinase transcriptional regulator transferred from obligate dehalorespiring Dehalobacter.

脱卤呼吸细菌利用有机卤化物作为电子受体，通过呼吸作用生长，并将有机卤化物还原为卤化程度较低的化合物。我还从不同的环境样本中富集和分离了三门在系统发育和功能上不同的脱盐呼吸细菌。其中，Geobacter sp. AY表现出前所未有的高脱氯活性，可在一周内将1,000 ppm的1,2-二氯乙烷（1,2- dca）解毒到乙烯。Geobacter sp. AY在生物修复中具有以下优势：对高浓度1,2- dca的高活性，仅以乙酸为唯一电子供体和碳源的培养性，以及在没有任何有机卤化物的情况下培养的高活性。基因组分析显示，组成型表达可能是由于缺乏cAMP激酶转录调节因子的质粒从专性脱盐呼吸脱盐杆菌转移而来。

项目成果

Involvement of Dehalobacter strains in the anaerobic dechlorination of 2,4,6-trichlorophenol.

• DOI: 10.1016/j.jbiosc.2013.05.009
• 发表时间: 2013-11
• 期刊: Journal of bioscience and bioengineering
• 影响因子: 2.8
• 作者: Zhiling Li;D. Suzuki;Chunfang Zhang;N. Yoshida;Suyin Yang;A. Katayama

Anaerobic 4-chlorophenol mineralization in an enriched culture under iron-reducing conditions

铁还原条件下富集培养物中的厌氧 4-氯酚矿化

• DOI: 10.1016/j.jbiosc.2014.04.007
• 发表时间: 2014
• 期刊: Journal of Bioscience and Bioengineering
• 影响因子: 2.8
• 作者: Zhiling Li;Daisuke Suzuki;Chunfang Zhang;Suyin Yang;Jun Nan;Naoko Yoshida;Aijie Wang;Arata Katayama
• 通讯作者: Arata Katayama

A humin-dependent Dehalobacter species is involved in reductive debromination of tetrabromobisphenol A.

• DOI: 10.1016/j.chemosphere.2013.05.051
• 发表时间: 2013-08
• 期刊: Chemosphere
• 影响因子: 8.8
• 作者: Chunfang Zhang;Zhiling Li;D. Suzuki;L. Ye;N. Yoshida;A. Katayama

Enrichment of new candidate PCB dechlorinator, Dehalobacter sp. FTH1 and the physiological and genomic characterization

新候选 PCB 脱氯剂 Dehalobacter sp 的富集。

• 发表时间: 2013
• 作者: Zhiling Li;Yasushi Inoue;Takuya Mizoguchi;Yohei Simizu;Naoko Y oshida andArata Katayama;増岡さゆり,濱岡裕美,段野正裕,入江朋子,佐能正剛,清水良,北村繁幸,太田茂,杉原数美;玉置仁，福田民治，中井智司，村岡大祐;Naoko Yoshida;石川宏美,田崎智也,田山剛崇,清水良,杉原数美;鈴木まゆみ，小瀬知洋，玉置仁，川田邦明;塚田真妃,田島慎也,渡部容子,浦丸直人,杉原数美,清水良,北村繁幸;Naoko Yoshida
• 通讯作者: Naoko Yoshida

多様な芳香族塩素化合物を脱塩素化するDehalobacter sp. FTH1株の ドラフトゲノム解析に基づく脱ハロゲン化酵素遺伝子解析

基于 Dehalobacter sp. FTH1 菌株基因组草图分析的脱卤酶基因分析，可对各种芳香族氯化合物进行脱氯。

• 作者: 吉田奈央子;Hauke Smidt;片山新太
• 通讯作者: 片山新太