Genomic and physiological characterization of dehalorespiring bacteria in three phyla

脱卤菌利用有机卤化物作为电子受体，通过呼吸作用生长，并将有机卤化物还原脱卤化为较少的卤化化合物。我还从不同的环境样本中丰富和分离了三个门在系统发育和功能上不同的脱盐呼吸细菌。其中，地杆菌属(Gebacter sp.)AY显示出前所未有的高脱氯活性，可在一周内将1000ppm的1，2-二氯乙烷(1，2-DCA)脱毒为乙烯。地杆菌属。在生物修复方面，AY具有高浓度1，2-DCA的高活性，仅以乙酸酯为唯一电子供体和碳源的可培养性，以及在不含任何有机卤化物的情况下生长的高活性。基因组分析表明，这种结构性表达可能是由专性脱盐呼吸脱卤杆菌转导的缺乏cAMP激酶转录调控因子的质粒所致。

Dehalorespiring bacteria grow by respiration using organohalides as electron acceptors and reductively dehalogenate organohalides to less halogenated compounds. I have also enriched and isolated phylogenetically and functionally different dehalorespiring bacteria of the three phyla from various environmental samples. Among them, Geobacter sp. AY showed unprecedented high dechlorination activity that detoxify 1,2-dichloroethane (1,2-DCA) of 1,000 ppm to ethene within a week. Geobacter sp. AY had following advantages to be applied in bioremediation; the high activity for high concentration of 1,2-DCA, culturability using only acetate as sole electron donor and carbon source, and the high activity in the culture grown without any organohalides. The genome analysis revealed the constitutive expression was probably resulted from a plasmid lacking of cAMP kinase transcriptional regulator transferred from obligate dehalorespiring Dehalobacter.