Plant Metabolism of Xenobiotics

外源物质的植物代谢

• 批准号: 5428147
• 负责人: Professor Dr. Erwin Grill
• 金额: --
• 依托单位: Lehrstuhl für Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5428147?language=en
• 关键词: Plant; Metabolism; Xenobiotics

Phytochelatin synthase not only catalyzes the heavy metal-dependent synthesis of phytochelatins but also serves as a catalyst for the key step in the turnover of lutathione conjugates in plants, the formation of g-glutamyl-cysteinyl conjugates. The latter function has recently been assigned by in vitro analyses. The project proposes now to test and corroborate the newly identified function of PCS in vivo. The analysis will focus on Arabidopsis by using plants, protoplasts and plant cell cultures. Mutants of Arabidopsis impaired in or lacking PCS will be analyzed for glutathione-conjugate turnover and phenotypic alterations. In addition, it is planned to transiently modulate phytochelatin synthase gene expression in order to assess its functions. The cellular compartmentation of both the reaction and of the reaction product will be examined including transport studies of the generated glutathioneconjugate derivative with isolated vacuoles.

植物螯合素合成酶不仅可以催化植物螯合素的重金属依赖性合成，而且还可以催化植物中胱甘肽偶联物转化的关键步骤，即g-谷氨酰半胱氨酸偶联物的形成。后一种功能最近已被体外分析确定。该项目现在建议在体内测试和证实新发现的PCS功能。分析将集中在拟南芥，利用植物，原生质体和植物细胞培养。拟南芥中受损或缺乏PCS的突变体将被分析谷胱甘肽偶联物的转换和表型改变。此外，计划瞬时调节植物螯合素合成酶基因表达以评估其功能。将检查反应和反应产物的细胞区隔，包括生成的谷胱甘肽偶联衍生物与孤立液泡的运输研究。