Active biomass in the marine subsurface tracked by intact polar membrane lipids

ODP/IODP的一个主要目标是获得对深埋沉积物中原核生物的性质和控制因素的详细了解。我们提出从深埋沉积物中完整极性脂质编码的结构和同位素信息中提取微生物生态和原核生物代谢信息。完整的极性脂质（ipl）是一种普遍存在的膜化合物，在死亡后迅速衰变，因此可以选择来自沉积物历史早期点的化石脂质信号。通过研究ODP第201、204和207段的沉积物，我们将解决两个首要的研究问题：(1)沉积物的地球化学状态与ipl的结构多样性和丰度之间的关系是什么？(2)对于深埋原核生物的代谢途径，从大块IPL组分的下核碳同位素剖面可以推断出哪些信息？分子同位素数据将与来自国家和国际合作者的互补分子遗传学数据相结合。该项目旨在资助一名博士生。谁将与德国和美国的合作者组成一个国际团队

One primary goal of ODP/IODP is to obtain a detailed understanding of the nature of and the factors that control prokaryotic life in deeply buried sediments. We propose to extract information on the microbial ecology and the metabolism of prokaryotes from the structural and isotopic information encoded in intact polar lipids in deeply buried sediments. Intact polar lipids (IPLs) are ubiquitous membrane compounds that rapidly decay after death and thus select against fossil lipid signals that originated from an earlier point in the sediment's history. Studying sediments from ODP Legs 201, 204, and 207, we will address the two overarching research questions: (1) What ist the relationship between the sediments' geochemical regime and the structural diversity and abundance of IPLs? (2) What information regarding the metabolic pathways utilized by deeply buried prokaryotes in-situ can be inferred from down-core profiles of the carbon-isotopic compositions of bulk IPL fraction? The molecular-isotopic data will be integrated with complementary molecular-genetic data from national and international collaborators. The project seeks to support a PhD student... who will be integrated an international team of collaborators in Germany and the U.S.