Characterisation and sub-cellular localisation of enzymes of the DOXP pathway inPlasmodium falciparum

恶性疟原虫 DOXP 途径酶的表征和亚细胞定位

• 批准号: 5436184
• 负责人: Dr. Jochen Wiesner
• 金额: --
• 依托单位: Biochemisches Institut
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5436184?language=en
• 关键词: Characterisation; sub; cellular; localisation; enzymes

In previous studies we demonstrated that in P. falciparum isoprenoids are synthesised by the 1-deoxy-D-xylulose 5-phosphate (DOXP) pathway. This pathway was discovered only recently and had not been fully elucidated at the beginning of our work. During the first two years funding period we were able to functionally characterise the last two missing enzymes, GcpE and LytB. Both enzymes turned out to represent oxygen-sensitive [4Fe-4S] proteins. Although not fully proven, there is evidence that the biochemical reactions of the DOXP pathway may take place inside the plastid-like organelle (apicoplast) of P. falciparum. Therefore, we plan to study the sub-cellular localisation of the involved enzymes. In addition, we plan to identify additional apicoplast-resident proteins required as co-factors for the enzymatic activity of GcpE and LytB. We expect that these studies will contribute to our understanding of the metabolic functions of the apicoplast.

在以前的研究中，我们证明了在恶性疟原虫中，类异戊二烯是通过1-脱氧-D-木酮糖5-磷酸（DOXP）途径合成的。这条途径是最近才发现的，在我们的工作开始时还没有完全阐明。在前两年的资助期间，我们能够在功能上取代最后两种缺失的酶，GcpE和LytB。这两种酶都是氧敏感的[4Fe-4S]蛋白。虽然尚未完全证实，但有证据表明DOXP途径的生化反应可能发生在恶性疟原虫的质体样细胞器（顶质体）内。因此，我们计划研究所涉及的酶的亚细胞定位。此外，我们计划确定额外的顶质体驻留蛋白作为GcpE和LytB的酶活性的辅因子。我们希望这些研究将有助于我们了解顶质体的代谢功能。