マイクロ流体過程を用いた効果的なクルクミン送達のためのポリエステルナノ粒子の合成

所提出的研究目标顺利完成。一种新型的微流体辅助纳米沉淀法，能够合成无表面活性剂的姜黄素包被聚（乳酸-羟基乙酸酯）纳米颗粒（curl - plga NP），其粒径可调（30-70 nm），粒径分布窄（多分散指数小于0.2）。这些结果证实了微流控装置在开发用于药物递送的新型聚酯纳米颗粒中是有用的。除了对合成的Cur- PLGA NPs进行物理表征外，还利用紫外可见光谱对其在溶液中的降解动力学进行了研究。结果表明，这些Cur- PLGA NPs在数天内是稳定的，此外，包封的姜黄素分子在水环境中具有显著的溶解和稳定作用。我们的curl - plga NP具有优异的胶体稳定性和抑制姜黄素的降解。此外，体外细胞研究的结果表明，我们的curl - plga NP作为姜黄素的有效递送系统的潜力。我们的微流体辅助方法为姜黄素提供了一种可控、有效的纳米生物材料合成给药系统，可扩展到不同的胶囊材料，用于各种生物医学应用。这些研究结果已在国际研讨会和会议上发表，并已在国际同行评审期刊上发表。

The proposed research goals are completed smoothly as expected. A novel microfluidic assisted nanoprecipitation strategy that enables synthesis of surfactant-free curcumin encapsulated poly(lactide-co-glycolide) nanoparticles (Cur-PLGA NP) with adjustable particle diameters (30-70 nm) and narrow particle size distribution (polydispersity index less than 0.2). These results confirm that microfluidic devices for are useful in the development of novel polyester nanoparticles for drug delivery. In addition to the physical characterization of the synthesized Cur- PLGA NPs, its degradation kinetics in solution was investigated using UV-vis spectroscopy. The results indicated that these Cur- PLGA NPs are stable for days, furthermore, the encapsulated curcumin molecules are significantly solubilized and stabilized in an aqueous environment. Our Cur-PLGA NP exhibit excellent colloidal stability and inhibit degradation of curcumin. Furthermore, results from in vitro cell studies demonstrate the potential of our Cur-PLGA NP as an effective delivery system for curcumin. Our microfluidic assisted approach offers a controlled and effective nano-biomaterials synthesis of drug delivery system for curcumin, which can be extended to different capsule materials for a variety of biomedical applications. These results have been presented in international symposium and conference and has been published in an international peer-reviewed journal.