Genome-wide analysis of multidrug efflux in the plant-pathogenic bacterium Pseudomonas syringae

植物病原菌丁香假单胞菌多药流出的全基因组分析

• 批准号: 5453528
• 负责人: Professor Dr. Matthias S. Ullrich
• 金额: --
• 依托单位: Department of Life Sciences and Chemistry
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5453528?language=en
• 关键词: Genome; wide; analysis; multidrug; efflux

Multidrug efflux (MDE) transporters are major contributors to bacterial resistance towards antibiotics. In contrast to the well-understood role of MDE in clinically relevant microbes, only little is known about MDE transporters in environmental bacteria. This project aims to identify and characterize all MDE pumps in the plant pathogen Pseudomonas syringae and to gain in-depth knowledge about their regulation and natural functions. MDE pumps may play an important role in the adaptation of P. syringae to its respective host plants by protecting them against plant antimicrobials. P. syringae is sub-divided into pathogenic variants (pathovars) which vary according to their host range. Available genome sequences of three different P. syringae pathovars will be used to develop a microarray containing genes of all members of transport protein families that include MDE systems. The microarrays will be used to identify transporters that are expressed after treatment with antibiotics, antimicrobial plant metabolites, and in planta. The to-be-tested P. syringae strains infect plants belonging to different plant families, each producing a different spectrum of antimicrobial metabolites. Thus, the diversity of plantborne metabolites will be screened as substrates of MDE transporters potentially shedding light on the contribution of MDE systems on host specificity. Putative MDE proteins will be cloned to determine substrate specificity, to search for natural substrates, and to study their transcriptional regulation. Because not all MDE transporters might show an increased expression in response to antibiotics, we will furthermore clone and DFG Research proposal - Matthias Ullrich - Multidrug efflux in plant pathogens 3 characterize genes that show sequence similarities to known MDE pumps from other organisms. Mutagenesis will be used to reveal the role of these transporters during pathogenesis.

多药外排（MDE）转运蛋白是细菌对抗生素耐药性的主要贡献者。与MDE在临床相关微生物中的作用相比，对环境细菌中的MDE转运蛋白知之甚少。该项目旨在识别和表征植物病原体假单胞菌中的所有MDE泵，并深入了解其调节和自然功能。MDE泵可能通过保护它们免受植物抗菌剂的侵害，在P. dichloringae适应其各自的宿主植物方面发挥重要作用。P. pastingae被细分为致病性变体（pathovars），其根据其宿主范围而变化。将使用三种不同的P. pathovars的可用基因组序列来开发含有包括MDE系统的转运蛋白家族的所有成员的基因的微阵列。微阵列将被用来识别转运蛋白后表达的抗生素，抗菌植物代谢产物，并在植物。待测试的丁香假单胞菌菌株感染属于不同植物科的植物，每种植物都会产生不同谱的抗菌代谢产物。因此，植物代谢物的多样性将被筛选为MDE转运蛋白的底物，可能揭示MDE系统对宿主特异性的贡献。将克隆推定的MDE蛋白以确定底物特异性，寻找天然底物，并研究其转录调控。由于并非所有MDE转运蛋白都可能在对抗生素的反应中表现出增加的表达，因此我们将进一步克隆和DFG研究提案- Matthias Ullrich -植物病原体中的多药外排3表征与来自其他生物体的已知MDE泵显示序列相似性的基因。突变将用于揭示这些转运蛋白在发病过程中的作用。