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Studies on cell-by-cell color variation of hydrangea

绣球花细胞颜色变化的研究

基本信息

批准号：
21K19216
负责人：
吉田久美
金额：
$ 4.08万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Research (Exploratory)
财政年份：
2021
资助国家：
日本
起止时间：
2021-07-09 至 2024-03-31
项目状态：
已结题

项目摘要

一般にアジサイの園芸品種には青色品種と赤色品種の区別があるとされ、青色品種を中性土壌で栽培しても赤色ではなく紫色までしか色は変化しない。また、赤色品種でも同様で、酸性土壌で栽培しても青色は呈さず、紫色のガク片になる。さらに、紫色のアジサイは、着色細胞が青、紫、赤色のモザイク状で、当研究室でそれぞれの色を細胞成分の分析が行われた結果、発色にはアルミイウムイオン（Al3+）と5-アシル化キナ酸（5CQ, 5pCQ）のデルフィニジン3-グルコシド（Dp3G）に対する当量比が影響することが明らかになった。ところが、加茂花鳥園で育種されたアジサイ品種「ごきげんよう」は同一品種で栽培条件の違いによって美しい青色と赤色とが発色する珍しい品種である。そこで、このアジサイのガク片の違いによる有機・機成分の違いを分析することで、発色の違いを解明しようと考えた。当初は、ガク片をプロトプラスト化して有機成分と無機成分を分析しようと計画したが、適当なプロトプラスト化の条件を見いだすことができなかったため、ガク片全体での分析を実施した。ごきげんようでは、青色に発色させた時の方がアントシアニン量がガク片の新鮮重量あたりでは3倍程度高かった。その結果、5アシル化キナ酸量（5CQ 2と5pCQ 3の合計量）が新鮮重量あたりの絶対量では青色と赤色で同程度であったものの、Dp3Gに対する当量で計算すると青色で低い結果となった。ガク片のMSイメージングより、Dp3GとAl3+は二層目の着色細胞だけに、助色素は表層と二層目の着色細胞に局在する。ごきげんようでも同様の分布を仮定すれば、Dp3Gに対するAlの当量は約6当量と、試験管内での色再現実験からも、十分に青色が発色する条件を満たす。ごきげんようの発色の違いは、栽培条件によるAl量の違いだけに起因することがわかった。

The difference between green and red varieties is that green varieties are cultivated in neutral soil and purple varieties are cultivated in neutral soil. The red variety is the same color, the acid soil is the same color, the purple is the same color, and the purple is the same color. In the laboratory, the color of the cells was analyzed. The results showed that the color of the cells was different from that of the blue cells. The color of the cells was different from that of the purple cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the red cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the red cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the red cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the red cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the red cells. The color of the cells was different from that of the blue cells. The color of the cells was different from that of the red cells. The color of the cells was different from that of The same variety is cultivated in violation of the same cultivation conditions. Analysis of organic and organic components in organic and inorganic compounds Analysis of organic and inorganic components in the first phase of the process The weight of the fresh tablet is 3 times higher than that of the original tablet. The result is that the amount of acid (5CQ2 and 5PCQ3) is the same as the amount of fresh weight. The amount of acid is the same as the amount of green weight. The amount of acid is the same as the amount of green weight. The amount of acid is the same as that of green weight. The amount of acid is the same as that of green weight. The amount of acid is the same as that of green weight. The color cells of the two layers of Dp3G and Al3 + are separated, and the color cells of the two layers of Dp3G and Al3 + are separated The color distribution of Dp 3G is about 6 equivalents. The color reproduction of Dp 3G is about 6 equivalents. The color of the rice is not consistent with the cultivation conditions.