Localization and intracellular trafficking of the chemokine receptor CCR7 after ligand mediated signal transduction and chemotaxis

趋化因子受体CCR7指导T细胞和成熟树突状细胞(DC)向次级淋巴器官的迁移。借助CCR7-GFP、CCR7-YFP和CCR7-CFP，我们将研究CCR7在迁移过程中的极化和细胞内转运。FRET测量和共聚焦显微镜将用于确定与Reggie-1/2、GPI锚定蛋白和PI-3，4，5-P3的共定位。C末端截断突变体和赖氨酸替换突变体将用于确定内吞作用、信号转导和迁移的信号。PGE2调节的基因和CCR7循环在体内迁移中的作用将被调查。N-末端标记的CCR7将用于识别与受体相关的信号复合体。

The chemokine receptor CCR7 directs the migration of T cells and mature dendritic cells (DC) into secondary lymphoid organs. With the help of CCR7-GFP, CCR7-YFP, and CCR7-CFP transfectants we will investigate the polarization and intracellular trafficking of CCR7 during migration. FRET measurements and confocal microscopy will be used to determine colocalisation with reggie-1/2, GPI-anchored proteins, and Pi-3,4,5-P3. C-terminal truncation variants and lysine replacement mutants will be used to determine signals for endocytosis, signal transduction, and migration. The role of PGE2-regulated genes and CCR7 recycling for in vivo migration will be investigated. N-terminally tagged CCR7 will be used to identify receptor associated signaling complexes.