Localization and intracellular trafficking of the chemokine receptor CCR7 after ligand mediated signal transduction and chemotaxis

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The chemokine receptor CCR7 directs the migration of T cells and mature dendritic cells (DC) into secondary lymphoid organs. With the help of CCR7-GFP, CCR7-YFP, and CCR7-CFP transfectants we will investigate the polarization and intracellular trafficking of CCR7 during migration. FRET measurements and confocal microscopy will be used to determine colocalisation with reggie-1/2, GPI-anchored proteins, and Pi-3,4,5-P3. C-terminal truncation variants and lysine replacement mutants will be used to determine signals for endocytosis, signal transduction, and migration. The role of PGE2-regulated genes and CCR7 recycling for in vivo migration will be investigated. N-terminally tagged CCR7 will be used to identify receptor associated signaling complexes.