Monitoring diversity patterns of active flagellate taxa in different grassland ecosystems as a tool in terrestrial ecology

作为陆地生态学的工具，监测不同草原生态系统中活跃鞭毛类群的多样性模式

• 批准号: 61949274
• 负责人: Professor Dr. Antonis Chatzinotas
• 金额: --
• 依托单位: Arbeitsgruppe Biodiversität
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/61949274?language=en
• 关键词: Monitoring; diversity; patterns; active; flagellate

Protist ecology thus far has focused nearly exclusively on functional group models, such as heterotrophic nanoflagellates. Recent investigations, however, indicate high molecular diversity and functional diversification, and habitat specificity of distinct flagellate taxa. The goals of the project are to correlate molecular diversity patterns of selected flagellate taxa (cercomonads, kinetoplastea, chrysomonads) with vegetation type and land use. The overwhelming majority of published DNA-based studies targets the potential diversity of protists since non-active, encysted cells are analyzed with this approach as well. Contrary to this, we will apply and evaluate adequate and specific tools to identify active members of these protistan taxa (i.e. the realized activity). Therefore, we will focus on 18S rRNA-based approaches as a measure to target potentially active flagellate organisms. Ribosomal RNA targeting primers will be used to specifically quantify with quantitative PCR the rRNA of selected flagellate groups in soil samples. The underlying diversity will be investigated on a broader level with a fingerprinting method (T-RFLP), whereas more detailed information shall be obtained by establishing clone libraries. In addition, we aim to develop and apply a rapid quantification tool for soil protist cells combining multiparametric flow cytometry with specific phylogenetic staining. In sum, we aim in particular to establish diversity patterns of ubiquitous active soil flagellates as a monitoring tool for landscape ecology.

到目前为止，原生生物生态学几乎完全集中在功能群模型上，例如异养纳米鞭毛虫。然而，最近的调查表明，高分子多样性和功能多样化，不同的鞭毛虫类群的栖息地特异性。该项目的目标是将选定的鞭毛虫分类群（尾单胞菌、动质体、金单胞菌）的分子多样性模式与植被类型和土地利用相关联。绝大多数已发表的基于DNA的研究针对原生生物的潜在多样性，因为非活性的包囊细胞也用这种方法进行分析。与此相反，我们将应用和评估适当的和具体的工具，以确定这些原生生物类群的活跃成员（即实现的活动）。因此，我们将专注于基于18 S rRNA的方法作为针对潜在活性鞭毛虫生物的措施。核糖体RNA靶向引物将用于通过定量PCR对土壤样品中选定鞭毛虫群体的rRNA进行特异性定量。将采用指纹法（T-RFLP）在更广泛的层面上调查潜在的多样性，而更详细的信息将通过建立克隆文库获得。此外，我们的目标是开发和应用土壤原生生物细胞的快速定量工具相结合的多参数流式细胞术与特定的系统发育染色。总之，我们的目标是特别是建立多样性模式的普遍存在的活性土壤鞭毛虫作为景观生态学的监测工具。