ラマン・蛍光イメージングフローサイトメトリーとセルソーティング

拉曼/荧光成像流式细胞仪和细胞分选

• 批准号: 21J15001
• 负责人: LINDLEY Matthew Mark
• 金额: $ 0.96万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-28 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21J15001/
• 关键词: Raman; Cell Sorting; Label-free

The goal of this project was to establish simultaneous Raman & fluorescence cell sorting based & imaging flow cytometry. This required combined development in three fields: nonlinear spectroscopy, microfluidics, and real-time data processing and decision making. At the time of this report, I have achieved cell sorting based on analysis of broadband, fingerprint region Raman spectra at throughputs of up to ~50 cells/s and purities up to 93%. This work required constructing the cell sorter, a proof of concept sorting using the Raman signal from polymer microbeads, and sorting cells based on the Raman signal from their polysaccharide and carotenoid content. A manuscript based on the results is currently under review.

本项目的目标是建立基于拉曼-荧光细胞同时分选的成像流式细胞术。这需要在三个领域进行联合开发：非线性光谱学、微流体学以及实时数据处理和决策。在撰写本报告时，我已经实现了基于宽带分析的细胞分选，指纹区域拉曼光谱的吞吐量高达~50个/S，纯度高达93%。这项工作需要构建细胞分选器，使用来自聚合物微珠的拉曼信号进行概念分选，并根据细胞的多糖和类胡萝卜素含量的拉曼信号对细胞进行分选。目前正在审查一份基于调查结果的手稿。

项目成果

High-throughput Raman-activated on-chip cell Sorting

高通量拉曼激活片上细胞分选

• 发表时间: 2021
• 作者: Lindley Matthew;Gala de Pablo Julia;Kinegawa Ryo;Hiramatsu Kotaro;Goda Keisuke;Matthew Mark Lindley
• 通讯作者: Matthew Mark Lindley

Highly sensitive Fourier-transform coherent anti-Stokes Raman scattering spectroscopy via genetic algorithm pulse shaping

• DOI: 10.1364/ol.434054
• 发表时间: 2021-09-01
• 期刊: OPTICS LETTERS
• 影响因子: 3.6
• 作者: Lindley, Matthew;Gala de Pablo, Julia;Goda, Keisuke
• 通讯作者: Goda, Keisuke