Central project

中央项目

• 批准号: 76388336
• 负责人: Professor Dr. Ekkehard Neuhaus
• 金额: --
• 依托单位: Fachgebiet Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/76388336?language=en
• 关键词: Central; project

This research group will focus on the analysis of processes involved and governing the dynamic storage functions of plant vacuoles during cold and osmotic stress. Since we represent a dislocated consortium of researchers the central service project has to provide plant material grown under highly controlled conditions and genetic material. Moreover, the central project will support all groups with various types of plant samples required for their individual projects. In addition, the laboratory in Kaiserslautern is well equipped with analytical devices allowing identification and quantification of all solutes with relevance to the consortium, e.g. primary metabolites like sugars, amino acids, low abundant metabolites and nutrients like nitrate, sulphate, phosphate and all metals (via ICAP). We apply for a half time position for a technician (E6/2) who will be responsible for plant growth under controlled conditions, for mutant plant propagation and crossing (including the required genetic analysis), for sample preparation and solute quantification, as well as for vacuole purification and subsequent membrane preparation. Support for central coordination costs is required for (i) upcoming regular biannual scientific meetings, (ii) exchange of co-workers among the participating laboratories with respect to running experiments, (iii) financing of at least two workshops, (iv) microarray analyses, (v) planning and organisation of an international scientific meeting, (vi) travel money for the whole research group and (vii) publication costs.

该研究小组将重点分析冷胁迫和渗透胁迫期间植物液泡动态储存功能所涉及的过程和控制过程。由于我们代表了一个混乱的研究人员联盟，因此中央服务项目必须提供在高度控制的条件下生长的植物材料和遗传材料。此外，中心项目将为所有团体提供其各自项目所需的各种类型的植物样本支持。此外，凯泽斯劳滕的实验室配备了精良的分析设备，可以识别和量化与联盟相关的所有溶质，例如主要代谢物，如糖、氨基酸、低丰度代谢物和营养物，如硝酸盐、硫酸盐、磷酸盐和所有金属（通过 ICAP）。我们申请半职技术员职位（E6/2），负责受控条件下的植物生长、突变植物的繁殖和杂交（包括所需的遗传分析）、样品制备和溶质定量，以及液泡纯化和随后的膜制备。需要对中央协调费用提供支持：(i) 即将举行的每半年一次的定期科学会议，(ii) 参与实验室之间就运行实验进行的同事交流，(iii) 资助至少两次研讨会，(iv) 微阵列分析，(v) 规划和组织国际科学会议，(vi) 整个研究小组的旅费和 (vii) 出版费用。