Energy transport in cells. Physiological functions and structural aspects of nucleotide transport proteins

细胞内的能量传输。

• 批准号: 128736536
• 负责人: Professor Dr. Ekkehard Neuhaus
• 金额: --
• 依托单位: Fachgebiet Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Reinhart Koselleck Projects
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/128736536?language=en
• 关键词: Energy; transport; cells.; Physiological; functions

All life on earth depends upon the availability of cellular energy in form of adenosine triphosphate(ATP). In cells, ATP is transported via two unrelated types of transport proteins,namely so called ADP/ATP carriers (AAC) belonging to the mitochondrial carrier family (MCF),and nucleotide transporters (NTT), residing either in plant plastids or in intracellular humanpathogenic bacteria. On one hand, our knowledge on the molecular structure and biochemistryof mitochondrial AAC proteins is impressive, but our understanding of the cellular function offurther nucleotide transporting MCF proteins is surprisingly poor. On the other hand, we knowmany aspects of the physiological function of NTT proteins in plants and bacteria, but wepossess limited information about the molecular structure of this type of carrier. The work proposedhere will help to uncover the function of many uncharacterized MCF type nucleotide transportersin plant cells. Moreover, I wish to identify the ATP transporter from the yeast EndoplasmicReticulum and I will elucidate the molecular structure of a first NTT protein. The outcomeof this research will further enlighten how cells transport ATP and derivates across theirmembranes and will provide insights into the structural determinants allowing ATP transport byNTT proteins.

地球上的所有生命都依赖于以三磷酸腺苷（ATP）形式存在的细胞能量。在细胞中，ATP通过两种不相关的运输蛋白进行运输，即属于线粒体载体家族（MCF）的ADP/ATP载体（AAC）和核苷酸转运蛋白（NTT），它们存在于植物质体或细胞内的人类致病菌中。一方面，我们对线粒体AAC蛋白的分子结构和生物化学的了解令人印象深刻，但我们对MCF蛋白核苷酸转运的细胞功能的了解却令人惊讶地贫乏。另一方面，我们知道植物和细菌中NTT蛋白的许多生理功能，但我们对这种载体的分子结构知之甚少。本文提出的工作将有助于揭示植物细胞中许多未表征的MCF型核苷酸转运蛋白的功能。此外，我希望从酵母内质网中鉴定ATP转运体，并阐明第一个NTT蛋白的分子结构。这项研究的结果将进一步启发细胞如何转运ATP及其衍生物跨膜，并将提供洞察结构决定因素允许ATP转运ntt蛋白。

项目成果

Identification of an Arabidopsis Plasma Membrane–Located ATP Transporter Important for Anther Development[W]

拟南芥质膜定位 ATP 转运蛋白对花药发育重要的鉴定[W]

• DOI: 10.1105/tpc.111.084574
• 发表时间: 1944
• 期刊: Plant Cell
• 影响因子: 11.6
• 作者: Rieder B;Neuhaus HE
• 通讯作者: Neuhaus HE

Nucleotides and Nucleosides: Transport, Metabolism, and Signaling Function of Extracellular ATP

• DOI: 10.1007/978-3-642-38797-5_4
• 发表时间: 2014
• 作者: T. Möhlmann;I. Steinebrunner;E. Neuhaus

Limitation of nocturnal ATP import into chloroplasts seems to affect hormonal crosstalk, prime defense, and enhance disease resistance in Arabidopsis thaliana.

夜间 ATP 输入叶绿体的限制似乎会影响拟南芥的激素串扰、主要防御和增强抗病性

• DOI: 10.1094/mpmi-02-10-0045
• 发表时间: 2010
• 期刊: Molecular plant-microbe interactions : MPMI
• 作者: Schmitz G;Reinhold T;Gobel C;Feussner I;Neuhaus HE;Conrath U
• 通讯作者: Conrath U

In vitro analyses of mitochondrial ATP/phosphate carriers from Arabidopsis thaliana revealed unexpected Ca2+-effects

• DOI: 10.1186/s12870-015-0616-0
• 发表时间: 2015-10-06
• 期刊: BMC PLANT BIOLOGY
• 影响因子: 5.3
• 作者: Lorenz, Andre;Lorenz, Melanie;Haferkamp, Ilka
• 通讯作者: Haferkamp, Ilka