Defining the role of the two vacuolar proton-pumps during stress responses

定义两个液泡质子泵在应激反应期间的作用

• 批准号: 76388002
• 负责人: Professorin Dr. Karin Schumacher
• 金额: --
• 依托单位: Forschungsgruppe Zellbiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/76388002?language=en
• 关键词: Defining; role; two; vacuolar; proton

Although it is generally assumed that the combined action of the vacuolar H+-ATPase (V-ATPase) and the vacuolar H+-pyrophosphatase (V-PPase) energizes transport across the tonoplast, their relative contribution to vacuolar and cytosolic pH-homeostasis during coldand osmotic stress has not been determined. During the first funding period, we have established that under non-stress conditions the V-PPase does not contribute to vacuolar acidification. Surprisingly, we found that in addition to the tonoplast V-ATPase, the endosomal VATPase seems to play an important role in vacuolar acidification and stress tolerance. Based on these results, focus will be on the function and regulation of the V-ATPase in the dynamic continuum of endomembrane compartments that ultimately delivers cargo to the central vacuole. In addition, we will continue our efforts to determine the role of the V-PPase under cold- and osmotic stress by using nanosensors for pH and key metabolites.

虽然一般认为液泡H ~+-ATPase（V-ATPase）和液泡H ~+-焦磷酸酶（V-PPase）的联合作用促进了液泡膜的运输，但它们在低温和渗透胁迫下对液泡和胞质pH稳态的相对作用尚未确定。在第一个资助期间，我们已经确定，在非应激条件下，V-PPase不会导致液泡酸化。令人惊讶的是，我们发现，除了液泡膜V-ATP酶，内体VATP酶似乎在液泡酸化和胁迫耐受性中发挥重要作用。基于这些结果，重点将放在功能和调节的V-ATP酶在动态连续的内膜室，最终提供货物的中央液泡。此外，我们将继续努力，通过使用pH和关键代谢物的纳米传感器来确定V-PPase在冷和渗透胁迫下的作用。

项目成果

pH in the plant endomembrane system-an import and export business.

• DOI: 10.1016/j.pbi.2014.09.005
• 发表时间: 2014-12
• 期刊: Current opinion in plant biology
• 影响因子: 9.5
• 作者: K. Schumacher