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Difference of relay modes in synaptic organization of the somatic and visceral thalamic nuclei.

体细胞和内脏丘脑核突触组织中继模式的差异。

基本信息

批准号：
08680810
负责人：
NAKAMURA Yasuhisa
金额：
$ 1.54万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680810/
关键词：
thalamus VL VA PVT MGN thalamic reticular nucleus axonal transport EM 3-D reconstruction 小脳脚内核

项目摘要

We observed light and electron microscopically somatic and visceral thalamic nuclei to know their characteristics of the synaptic organization. Wechose the ventral lateral (VL) and ventral anterior (VA) nuclei as the motor, the medial geniculate nucleus (MGN) as the sensory and the paraventricular thalamic nucleus (PVT) as the visceral nuclei.In the VL,three-dimensional reconstruction from serial ultrathin sections revealed that cerebellar terminals (CLRs) predominantly made synaptic contacts on primary and secondary dendrites of thalamocortical projection neurons (TCNs), whereas, cortical terminals made synapses on distal dendrites. Presynaptic dendrites (PSDs) of intrinsic neurons and axon terminals of unidentified origins (Ps) made synapses on every order of dendrites and neuronal somata. The synaptic complex (synaptic triad), formed by the CLR,dendrites of the TCN and the PSD was also analyzed. Terminals from the thalamic reticular nucleus (TRN), which belong to P were located independently from the synaptic triad. In the VA,entopeduncular terminals (ELPs) mainly made synaptic contacts with somata and primary dendrites of TCNs. The synaptic triad among the ELP,TCN dendrites and PSD was also observed. Because the ELP is inhibitory in nature, the PSD may play a role of disinhibition against the TCN.TRN terminals in the VA often showed serial synapses in which they were posynaptic to ELPs or terminals with round vesicles.We studied the corticocortical connections to know the morphological bases of auditory information processing. During this study we examined the relationship between auditory related cortical areas and the subnuclei of the MGN precisely.About the PVT,we proved that neurons in the Barrington's nucleus which is known as the micturition center sent axons to both the spinal cord and the PVT by axon collaterals. We also observed that many PVT neurons gave off axons to both the limbic cortex and nucleus accumbens by collaterals.

我们通过光和电子显微镜观察丘脑体细胞核和内脏丘脑核，以了解它们的突触组织特征。我们选择腹外侧核（VL）和腹前核（VA）作为运动核，内侧膝状核（MGN）作为感觉核，室旁丘脑核（PVT）作为内脏核。丘脑皮质投射神经元（TCN）的初级和次级树突上的突触接触，而皮质末梢在远端树突上形成突触。内在神经元的突触前树突（PSD）和来源不明的轴突末端（Ps）在树突和神经元胞体的每个阶上形成突触。还分析了由 CLR、TCN 树突和 PSD 形成的突触复合体（突触三联体）。丘脑网状核 (TRN) 的末端属于 P，其位置独立于突触三联体。在VA中，内脚末端（ELP）主要与TCN的体细胞和初级树突进行突触接触。还观察到 ELP、TCN 树突和 PSD 之间的突触三联体。由于ELP本质上是抑制性的，PSD可能对TCN起到去抑制作用。VA中的TRN末端经常表现出与ELP或具有圆形囊泡的突触末端的串行突触。我们研究了皮质连接，以了解听觉信息处理的形态学基础。在本研究中，我们精确地研究了听觉相关皮层区域与MGN亚核之间的关系。关于PVT，我们证明了被称为排尿中枢的Barrington核中的神经元通过轴突侧支向脊髓和PVT发送轴突。我们还观察到许多 PVT 神经元通过侧支向边缘皮层和伏核发出轴突。