an in vivo or in vitro expression of c-SRC gene in bone formation on materials.

c-SRC基因在材料上骨形成中的体内或体外表达。

• 批准号: 08680931
• 负责人: YOSHIKAWA Takafumi
• 金额: $ 1.6万
• 依托单位: FIRST DEPT.OF PATHOLOGY NARA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680931/
• 关键词: c-SRC; alkaline phosphatase; osteocalcin; osteogenesis; biomaterial; tissue engineering; SRC gene; Osteogenesis

We investigated in vitro and in vivo osteogenic ability of cultured marrow cells by gene expression analysis of alkaline phosphatase (ALP), osteocalcin (OC) and c-SRC.In the initial stage of bone formation by cultured marrow cells, ALP, OC and c-SRC mRNA could be detected in vivo and in vitro. These are useful as a marker of bone formation to obtain valuable information about osteogenic response of cells on biomaterials. We succeeded in production of cultured bone/ceramic constructs which has high osteogenic potential in vivo. A significant benefit of this method is that the construct can be made with only a small amount of aspirated marrow cells from aged patients with little host morbidity. The analyses of these genes showed that we can assemble an in vitro bone/ceramic construct, and due to the porous framework of the ceramic, the construct has osteogenic potential similar to that of autologous cancellous bone.

我们通过碱性磷酸酶（ALP）、骨钙素（OC）和c-SRC的基因表达分析来研究培养骨髓细胞的体外和体内成骨能力。在培养骨髓细胞成骨的初始阶段，体内和体外均可检测到ALP、OC和c-SRC mRNA。这些可用作骨形成的标记物，以获得有关细胞对生物材料的成骨反应的有价值的信息。我们成功生产了具有高体内成骨潜力的培养骨/陶瓷结构。该方法的一个显着优点是，只需从宿主发病率极低的老年患者中抽取少量骨髓细胞即可制备构建体。对这些基因的分析表明，我们可以在体外组装骨/陶瓷结构，并且由于陶瓷的多孔框架，该结构具有与自体松质骨相似的成骨潜力。

项目成果

A.Nemoto, T.Uemura, Y-K.Liu, T.Yoshikawa, T.Ushida, T.Tateishi: "Study of osteopontin involvement in osteoblast differentiation for allogeneic transplantation of in vitro cultured bone." Bioceramics. vol.11(eds R.Z.LeGeros, J.P.LeGeros). 281-284 (1998)

A.Nemoto、T.Uemura、Y-K.Liu、T.Yoshikawa、T.Ushida、T.Tateishi：“体外培养骨同种异体移植中骨桥蛋白参与成骨细胞分化的研究”。

H.Nakajima, T.Yoshikawa, H.Ohgushi, M.Akahane, S.Tamai, K.Mishima and K.Ichijima: "Histological evaluation of cultured bone graft using cryopreserved marrow cells" Bioceramics. vol.10. 109-112 (1997)

H.Nakajima、T.Yoshikawa、H.Ohgushi、M.Akahane、S.Tamai、K.Mishima 和 K.Ichijima：“使用冷冻保存的骨髓细胞进行培养骨移植的组织学评估”生物陶瓷。

T.Yoshikawa et.al: "Analysis of gene expression in osteogenic cultured marrow/hydroxyapatite construct implanted at ectopic sites." J.Biomed.Mater.Res.41. 568-573 (1998)

T.Yoshikawa 等人：“异位植入的成骨培养骨髓/羟基磷灰石结构中的基因表达分析”。

T.Yoshikawa et al: "Morphological study on the meneralization of collgen fibers in in vitro bone formation" J.Japanese society for biomaterials. 16. 72-77 (1998)

T.Yoshikawa等人：“体外骨形成中胶原纤维矿化的形态学研究”J.日本生物材料学会。

K.Mishima, H.Matsuoka, H.Nakajima, T.Yoshikawa, H.Ohgushi, S.Tabata, E.Yamada and K.Ichijima: "Increased expression of extracellular signal regulated kinase, after axotomy in dorsal motor nucleus of the vagus nerve and the hypoglossal nucleus" Brain Res.7

K.Mishima、H.Matsuoka、H.Nakajima、T.Yoshikawa、H.Ohgushi、S.Tabata、E.Yamada 和 K.Ichijima：“迷走神经背运动核轴突切除术后，细胞外信号调节激酶的表达增加