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Bone and skin regeneration therapy with bone marrow mesenchymal cells

骨髓间充质细胞的骨和皮肤再生治疗

基本信息

批准号：
15500319
负责人：
YOSHIKAWA Takafumi
金额：
$ 2.37万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500319/
关键词：
bone skin marrow cell regeneration Dexamethasone estriol therapy Bone

项目摘要

Autogenous bone graft is ideal from the point of view of rejection and osteogenic potential, and also produces the best results clinically. When pseudoarthrosis occurs due to poor union of a bone fracture, cancellous bone with high cellular activity is generally harvested from the ilium and then grafted to the affected area to facilitate bone union. However, autogenous bone cannot be used to reconstruct extensive bone defects, as there is naturally a limit to the amount that can be harvested. This method also has the drawback of causing damage to normal bone and soft tissue. Harvesting cancellous bone from the ilium is associated with severe postoperative pain, and patients experience more pain at the harvest site than at the graft site, thus resulting in poor patient satisfaction. Management of pain at the harvest site is often difficult for patients and medical professionals. There have also been reports of pelvic deformation and persistent pain lasting more than one year after surge … More ry. Autograft skin flap operation is also invasive and unsuitable for old patients. If a tissue engineering approach was used to produce autogenous bone or skin ex vivo or with culture techniques, extensive skin or bone tissue defects could be repaired without damaging normal tissues.Bone marrow cells include hemopoietic cells and cells with an bone or skin tissue regeneration capacity. However, the ability of human marrow cells is unreliable. We have developed the culture techniques of human marrow mesenchymal cells to enhance its tissue regeneration ability. We have experienced more than 35 cases who were treated using our tissue engineering techniques. In all patients, autologous marrow mesenchymal cell transplantation was shown to be therapeutically effective, and there were no adverse reactions associated with transplantation. With present type of tissue-engineered bone or skin, an artificial material with a high tissue regeneration capacity can be prepared by aspiration, which is minimally invasive, and thus when compared to autografts, it is possible to radically reduce postoperative pain without damage of normal tissue. Less

从排异反应和成骨潜力的角度来看，自体骨移植是理想的，也是临床效果最好的。当由于骨折愈合不良而发生假关节时，通常从髂骨采集具有高细胞活性的松质骨，然后移植到患处以促进骨愈合。然而，自体骨不能用于重建广泛的骨缺损，因为可以收获的数量自然是有限的。这种方法也有对正常骨骼和软组织造成损伤的缺点。从髂骨取松质骨伴有严重的术后疼痛，患者在取骨部位比移植物部位感到更多的疼痛，因此导致患者满意度较低。对于患者和医疗专业人员来说，处理收获部位的疼痛通常是困难的。也有骨盆变形和持续疼痛超过一年的报道，自体皮瓣移植手术也有侵入性，不适合老年患者。如果使用组织工程方法在体外或通过培养技术产生自体骨或皮肤，则可以在不损伤正常组织的情况下修复广泛的皮肤或骨组织缺陷。骨髓细胞包括造血细胞和具有骨或皮肤组织再生能力的细胞。然而，人类骨髓细胞的能力是不可靠的。为了提高人骨髓间充质细胞的组织再生能力，我们开发了人骨髓间充质细胞培养技术。我们已经经历了超过35例使用我们的组织工程技术治疗的病例。在所有患者中，自体骨髓间充质细胞移植显示出治疗效果，并且没有与移植相关的不良反应。利用现有的组织工程骨或皮肤，可以通过抽吸制备具有高组织再生能力的人工材料，这是微创的，因此与自体移植物相比，可以从根本上减少术后疼痛而不损害正常组织。少