Research of tissue engineering using gene engineering technique

利用基因工程技术进行组织工程研究

• 批准号: 12680841
• 负责人: YOSHIKAWA Takafumi
• 金额: $ 2.37万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680841/
• 关键词: tissue engineering; gene; bone; wound; therapy; regeneration

Culture of bone marrow blood produces bone marrow mesenchymal cells that adhere to the bottom of the culture dish. These cells are known to include cells that can differentiate in various directions. Culturing these marrow mesenchymal cells with differentiation factors and biomaterials can achieve regeneration of bone and soft tissue. The present study showed that culture of marrow mesenchymal cells with porous artificial bone material in an osteogenic culture medium containing dexamethasone could produce new bone tissue on the artificial material. This bioartificial bone graft substitute could effectively regenerate bone tissue when it was implanted in vivo. Porous artificial bone material perfused with marrow mesenchymal cells also markedly stimulated bone regeneration using gene engineering technique. Similarly, when an artificial dermis made of collagen sponge was perfused with marrow mesenchymal cells and used to cover a skin defect, the regeneration of blood vessels was stimulated. Since the present results demonstrated the ability of marrow mesenchymal cells to regenerate bone and soft tissue, these cells are expected to be useful for tissue regeneration therapy.

骨髓血培养产生粘附于培养皿底部的骨髓间充质细胞。已知这些细胞包括可以向各个方向分化的细胞。用分化因子和生物材料培养这些骨髓间充质细胞可以实现骨和软组织的再生。本研究表明，在含有地塞米松的成骨培养基中培养骨髓间充质细胞与多孔人造骨材料，可以在人造材料上产生新的骨组织。这种生物人工骨移植替代品植入体内后可以有效地再生骨组织。利用基因工程技术，灌注骨髓间充质细胞的多孔人造骨材料也显着刺激了骨再生。同样，当用胶原海绵制成的人造真皮灌注骨髓间充质细胞并用于覆盖皮肤缺损时，血管的再生受到刺激。由于目前的结果证明骨髓间充质细胞具有再生骨和软组织的能力，因此预计这些细胞可用于组织再生治疗。

项目成果

T.Yoshikawa: "Bone reconstruction by cultured bone"Mat.Sci.Eng.C Biomim.. 13. 29-37 (2000)

T.Yoshikawa：“通过培养骨进行骨重建”Mat.Sci.Eng.C Biomim.. 13. 29-37 (2000)

J. lida, T. Yoshikawa, K. Ichijima, Y. Takakura: "Osteogenic potential of cultured bone/ceramic construct: a comparison with that of marrow mesenchymal cells/ceramic composite"Bioceramics. 14. 655-658 (2003)

J. lita、T. Yoshikawa、K. Ichijima、Y. Takakura：“培养骨/陶瓷结构的成骨潜力：与骨髓间充质细胞/陶瓷复合材料的比较”生物陶瓷。

Toshiaki Noshi, Takafumi Yoshikawa, Yoshiko Dohi, Masako Ikeuchi, Katsushiro Horiuchi, Kunio Ichijima, Masahito Sugimura, Kunio Yonemasu and Hajime Ohgushi,: "Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of mar

Toshiaki Noshi、Takafumi Yoshikawa、Yoshiko Dohi、Masako Ikeuchi、Katsushiro Horiuchi、Kunio Ichijima、Masahito Sugimura、Kunio Yonemasu 和 Hajime Ohgushi，：“重组人骨形态发生蛋白-2 增强了骨的体内成骨能力

T.Yoshikawa et al.: "In vivo osteogenic durability of cultured bone in porous ceramics."Transplantation. vol.69. 128-134 (2000)

T.Yoshikawa 等人：“多孔陶瓷中培养骨的体内成骨耐久性。”移植。

T.Noshi,T.Yoshikawa et al.: "Recombinant human bone morphogeneic protein-2 the in vivo osteogenic ability of marrow/HA composites."Artif.Organ. (in press).

T.Noshi、T.Yoshikawa 等人：“重组人骨形态发生蛋白-2 骨髓/HA 复合材料的体内成骨能力。”Artif.Organ。