メロンの新規性決定遺伝子の同定およびその育種利用

甜瓜新颖性决定基因的鉴定及其在育种中的应用

• 批准号: 22KJ0386
• 负责人: 梨木 聡人
• 金额: $ 1.09万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ0386/
• 关键词: 新規性決定遺伝子; メロン; 性表現

本課題では、雑草メロン系統‘UT1’に由来する新規性決定遺伝子（両性花性遺伝子および主枝成性遺伝子）の同定とその遺伝的メカニズムの全容解明に迫るとともに、その新規性決定遺伝子の育種利用における有用性を検証することを目標としている。令和4年度の具体的な研究内容とその成果は次の通りである。はじめに、‘UT1’の全ゲノム配列情報を取得し、既に解読済みの‘EF’と性決定遺伝子座上領域の塩基配列の比較を行った結果、両者の間に大きな構造変異は見られなかった。次に、2つの新規性決定遺伝子の育種利用における有用性を評価することを目的に、遺伝的背景が大きく異なる品種群に属する8品種・系統（花粉親）と雑草メロン系統（種子親）の交雑を行い、得られた雑種（F1）の性表現を調査した。その結果、全ての交配組合せで主枝成性を示したことから、‘UT1’に由来する主枝成性遺伝子は広範な品種群において容易に主枝成性を付与できる遺伝子として極めて有用であると考えられた。一方、花粉親として用いた8品種・系統における既報の性決定遺伝子（3遺伝子）の遺伝子型は全て同一であったが、F1の性表現は花粉親系統間で異なった。これは花粉親系統間における第8染色体に座上する両性花性遺伝子のアリルの違いに起因すると考えられた。第8染色体に座上する両性花性遺伝子については他の研究グループにより候補遺伝子が1つに絞られおり、花粉親の当該遺伝子配列を調査した結果、アリルと性表現の関係が一致しなかった。そのため、既報の性決定遺伝子との相互作用の詳細な解明を含む更なる研究が必要であると考えられた。

In this topic, the origin of the UT1' system, the new standard of sexual determination (sexual sex is the main branch of the disease) is the same as that of the same as that of the system. Ling and 4 years of "specific" research content, "results" and "general information". In this paper, the results of the data acquisition, the EF' decision and the results of the field-based configuration on the sub-seat are analyzed, and the results are analyzed. In the second and second century, it is decided that the seed breeding should make use of its usefulness, its purpose, its background, its background, and its background. It belongs to 8 kinds of systems (pollen plant), grass plant system (sub-plant), cross-breeding system (sub-plant), and it has been proved to be successful in the performance table of different varieties (F1). The results showed that the formation of the main branch of the whole mating system showed that the main branch of the whole mating group was easy to grow, and the main branch of the whole mating system showed that the formation of the main branch of the whole mating system showed that the formation of the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating system showed that the main branch of the whole mating group was easy, and the main branch of the whole mating system was very useful. On the one hand, 8 kinds of pollen were used in the pollen system, both the reported determinants (3) and the F _ 1 were all tested in the same cycle, and the F _ 1 sex table showed that the pollen system was in the same condition. In the pollen system, chromosome 8 is located on chromosome 8, and the cause of the disease is studied in the pedigree of the pollen system. Chromosome 8 is located on the pedestal. The sexual anther is not the same as the others. The pollen is the same as that of the pollen. It is necessary to study the necessary information about interaction, interaction, interaction and research.

项目成果

日本在来雑草メロンの性表現の遺伝的評価

日本本土杂草瓜性表达的遗传评价

• 发表时间: 2022
• 作者: 梨木聡人;吉岡洋輔
• 通讯作者: 吉岡洋輔

A novel femaleness allele of the sex determination gene CmACS11 in melon

甜瓜性别决定基因 CmACS11 的新雌性等位基因

• 发表时间: 2022
• 作者: 31.Nashiki Akito;Matsuo Hiroki;Fauziatul Fitriyah;Takano Kota;Isobe Sachiko;Shirasawa Kenta;Yoshioka Yosuke
• 通讯作者: Yoshioka Yosuke

雑草メロンに由来する主枝成性の遺伝的評価

杂草瓜主枝生长的遗传评价

• 发表时间: 2022
• 作者: 梨木聡人;吉岡洋輔
• 通讯作者: 吉岡洋輔