Developing new teaching materials to show the influences of Ultraviolet radiation on organisms for environmental education

开发新教材展示紫外线对生物体的影响，以进行环境教育

• 批准号: 09680205
• 负责人: MATSUDA Hitoshi
• 金额: $ 0.64万
• 依托单位: Osaka Prefectural Education Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680205/
• 关键词: Ultraviolet-radiation; Photoreactivation; Banana-pericarp; Yeast; duckweed (Spirodela); Drosophila; Xenopus; Environmental education; アフリカツメガエル; 教材開発; 紫外線; 致死効果; オゾンの効果

Stratospheric ozone depletion is a concern because the ozone layer in the stratosphere keeps 95-99% of the suns ultraviolet(UV) radiation from striking the earth. A number of consequences can result from increased levels of 1W striking the earth, including : genetic damage, eye damages and damage to marine life.To make students understand these rerelationships scientifically, we schemed out following methods in experiments, In the all experiments , we used a 1OW UV lamp(germicidal lamp) to apply UV light at 30cm.1. The pericarp of banana changed its yellow color to brown when it had received UV radiation for 2-3 minutes followed keeping in a dark room. This color change is available for the estimation of UV radiation. The tranamission factor of several materials for the UV radiation was studied by covering the surface of banana with the test materials.2. The one minute UV irradiation coused death in 99% of yeast cells, but the photorecovery was observed in the dying process. Using yeast cells as a material we can treat the potorecovery quantitatively.3. The thallus of duckweed (Spirodela punctata) was suppressed its growth by more than 20 min irrdiation of UV.The attacked thalluses lost its chlorophyl color gradually, and became white in color within a few days after the irradiation.4. In ampibian embryos (Xenopus laevis), UV irradiation over 50 minuts caused their abnormal cleavage, and then the living rate of them decreased at 36 hr after the irradiation. According to the prolongation of irradiation time, in the living embryos, the rate of embryos which developed abnormal form were increased.5. We made protocol of practical approach with the aspect of biological protection mechanism for (UV) radiation, and evaluate the importance to our environment and environment education.

平流层臭氧耗竭是一个问题，因为平流层中的臭氧层可使95-99％的太阳紫外线（UV）辐射阻止地球撞击。 1W击中地球的水平提高可能会导致许多后果，包括：遗传损害，眼睛损害和对海洋生物的损害。为了使学生科学地了解这些重新相关，我们在实验中采用了以下方法，在所有实验中，我们都使用了1级UV灯（真菌灯）以在30C上应用UV Light。香蕉的果皮在接受紫外线辐射2-3分钟后，在黑暗的房间里，它将其黄色变成了棕色。这种颜色变化可用于估计紫外线辐射。通过用测试材料覆盖香蕉的表面，研究了几种紫外线辐射的旋转因子。2。一分钟的紫外线辐照在99％的酵母细胞中造成了死亡，但在垂死过程中观察到了光回应。使用酵母细胞作为一种材料，我们可以定量处理POTOREDECONDY.3。 Duckweed（Spirodela Punctata）的thallus被抑制了20分钟以上的紫外线灌溉。受攻击的Thalluses逐渐损失其氯聚素，并在辐照后的几天内变成白色。4。在两座胚胎（Xenopus laevis）中，超过50张精制的紫外线辐射引起了异常的裂解，然后在辐照后36小时的生命速率降低。根据辐照时间的延长，在生物胚胎中，产生异常形式的胚胎速率增加了。5。我们制定了实用方法的协议，该方案与（紫外线）辐射的生物保护机制方面进行了评估，并评估了对我们的环境和环境教育的重要性。