Development of ultraviolet disinfection systems to reduce photoreactivation of microorganisms in water

开发紫外线消毒系统以减少水中微生物的光活化

• 批准号: 13555147
• 负责人: OHGAKI Shinichiro
• 金额: $ 7.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13555147/
• 关键词: ultraviolet inactivation; photoreactivation; Escherichia coli; Crvptosporidium parvum; Legionella pneumophila; low-pregsure UV lamp; medium-pressure UV lamp; ESS法; Escherichia coli; Crystosporidium parvum; ピリミジン二量体; 生残率測定法; 紫外線感受性

Ultraviolet inactivation and photoreactivation of Escherichia coli, Cryptosporidium parvum and Legionella pneumophila were compared using a low-pressure UV lam (LP ; 254nm) or a medium-pressure UV lamp (MP ; 220nm-580nm). Filtration of the MP emissions was also carried out to investigate the effect of wavelengths on the characteristics of inactivation and photoreactivation of microorganisms. An endonuclease sensitive site (ESS) assay was applied to determine the number of UV-induced pyrimidine dimers in the genomic DNA, while a conventional cultivation or infectivity assay was also used to investigate the viability of microorganisms.This research indicated that (l) C. parvum can be effectively inactivated by UV without photoreactivation, (2) MP lamp can repress photoreactivation of E. coli probably by affecting photolyase (3) L. pneumophila performs evident photoreactivation either after LP or MP lamp inactivation. These conclusions may encourage a development of UV systems with high disinfection efficiency at low photoreactivation risk.

用低压紫外灯(LP；254 nm)和中压紫外灯(MP，220 nm-580 nm)对大肠杆菌、微小隐孢子虫和嗜肺军团菌的紫外线灭活和光复活进行了比较。还对MP排放物进行了过滤，以考察波长对微生物灭活和光复活特性的影响。采用核酸内切酶敏感部位分析法检测紫外线诱导的嘧啶二聚体在基因组DNA中的数量，同时采用常规培养或感染力测定来研究微生物的生存能力。研究表明：(L)微小弧菌可以被紫外线有效灭活，而不需要光复活；(2)MP灯可能通过影响光解酶来抑制大肠杆菌的光复活；(3)嗜肺乳杆菌在LP灯或MP灯灭活后都表现出明显的光复活。这些结论可能会促进具有高消毒效率和低光复活风险的紫外线系统的发展。

项目成果

小熊久美子, 片山浩之, 三谷啓志, 大垣眞一郎: "エンドヌクレアーゼ・センシティブ・サイト法によるEscherichia coliの紫外線不活化および光回復の評価"日本水環境学会誌. 24・8. 534-538 (2001)

Kumiko Oguma、Hiroyuki Katayama、Keishi Mitani、Shinichiro Ogaki：“通过核酸内切酶敏感位点法评估大肠杆菌的紫外线灭活和光恢复”日本水环境学会杂志 24・8（2001 年）。

Oguma, K., H. Katayama, H. Mitani, S. Morita, T. Hirata, S. Ohgaki: "Determination of pyrimidine dimers in Escherichia coli and Cryptosporidium parvum during ultraviolet light inactivation, photoreactivation and dark repair"Applied and Environmental Micro

Oguma, K.、H. Katayama、H. Mitani、S. Morita、T. Hirata、S. Ohgaki：“在紫外光灭活、光再激活和暗修复过程中测定大肠杆菌和小隐孢子虫中的嘧啶二聚体”应用和环境微观

Oguma, K., H. Katayama, S. Ohgaki: "Determination of pyrimidine dimers in the genomic DNA of Escherichia coli during photoreactivation following inactivation by medium-pressure UV lamp"Journal of Japan Water Works Association. 72・4. 86-87 (2003)

Oguma, K., H. Katayama, S. Ohgaki：“中压紫外线灯灭活后光再激活期间大肠杆菌基因组 DNA 中嘧啶二聚体的测定”日本自来水协会杂志 72・4。 (2003)

K.OGUMA, H.KATAYAMA, S.OHGAKI: "Photoreactivation characteristics of Escherichia coli subsequent to inactivation by a low-pressure or a medium-pressure ultraviolet lamp"Appl.Environ.Microbiol.. 68・12. 6029-6035 (2002)

K.OGUMA、H.KATAYAMA、S.OHGAKI：“低压或中压紫外线灯灭活后大肠杆菌的光再活化特性”Appl.Environ.Microbiol.. 68・12. ）

Oguma, K., H.Katayama, S.Ohgaki: "Photoreactivation of Escherichia coli subsequent to low-or medium-pressure ultraviolet disinfection determined by an endonuclease sensitive site assay"Applied and Environmental Microbiology. 68・12. 6029-6035 (2002)

Oguma, K., H. Katayama, S. Ohgaki：“通过内切核酸酶敏感位点测定确定低压或中压紫外线消毒后大肠杆菌的光再活化”应用和环境微生物学 68・12。 ）