Release of mercury from dental amalgam fillings and behavior of the mercury in a living body

1. 妊娠大鼠牙汞合金填充物中汞的释放及汞在母体和胎儿组织中的分布。实验动物接受一次汞合金充填。在妊娠第2、8、15天连续24小时测量大鼠呼出的汞。动物在第2天显示出大量的汞。随着时间的推移，汞的含量趋于下降，但即使在第15天也观察到明显的汞含量。特别是在动物进食后，汞的含量增加。在母亲体内，汞浓度最高的部位是肾脏，其次是肺、肝、胎盘和大脑，依次递减。胎儿器官中汞浓度最高的是肝脏，其次是肾脏和大脑。孕妇脏器和胎儿肝脏中汞浓度显著高于对照组，孕妇全血、红细胞、血浆和胎儿全血中汞浓度也显著高于对照组。胎儿脑、肝、肾和全血中汞浓度低于母体组织。怀孕大鼠汞合金填充物中汞的释放及血液和尿液中的汞含量研究将20只大鼠平均分为四组（A、B、C和D组）。B、C、D组分别在1、2、4颗上颌磨牙行汞合金充填。A组不进行治疗。在妊娠第2、8、15天连续24小时测量大鼠呼出的汞。每隔2天采集一次尿样。随着时间的推移，汞的含量趋于下降，但即使在第15天也观察到明显的汞含量。血液样本是直接从心脏抽取的。用个人计算机计算汞合金面积。母鼠全血、血浆和尿液中汞的浓度与汞合金面积和大鼠呼出的汞蒸气有关。汞合金充填大鼠组织中汞的组织学观察。雌性大鼠20只作为实验动物，对照组10只。实验动物接受4次汞齐充填。在实验动物的大脑和肝脏切片中，未发现汞沉积，而在肾脏中发现了最大的汞沉积。汞的密集堆积主要位于近端小管细胞。少

1. Release of Mercury from Dental Amalgam Fillings in pregnant Rats and Distribution of Mercury in Maternal and Fetal Tissues.Experimental animals received one amalgam filling. On days 2, 8 and 15 of pregnancy, Hg exhaled by the rat was measured serially for 24 hours. The animals showed large amounts of Hg on day 2. The amount of Hg tended to decline as time elapsed but marked amounts were observed even on day 15. The amount of Hg increased particularly after the animal took food. In mothers, the highest Hg concentration was found in the kidneys followed, in decreasing order, by the lungs, liver, placenta and brain. The highest Hg concentration among fetal organs was found in the liver, followed by the kidneys and brain. Hg concentrations in maternal organs and fetal liver were significantly higher than those of the controls, and concentrations in maternal whole blood, erythrocyte and plasma, and fetal whole blood were also significantly higher. Hg concentrations in the fetal brain, li … More ver, kidneys and whole blood were lower than those of the maternal tissues.2. Release of Mercury from Dental Amalgam Fillings in Pregnant Rats and Mercury Contents in Their Blood and Urine SamplesTwenty rats were equally divided into four groups (Groups A, B, C and D). Groups B, C and D received amalgam fillings in 1, 2 and 4 maxillary molars, respectively. Group A received no treatment. On days 2, 8 and 15 of pregnancy, Hg exhaled by the rat was measured serially for 24 hours. Urine samples were collected at intervals of 2 days. The amount of Hg tended to decline as time elapsed but marked amounts were observed even on day 15. Blood samples were drawn directly from the heart. The amalgam area was calculated using a personal computer. Hg concentrations in maternal whole blood, plasma and urine correlated to the amalgam area and Hg vapor exhaled by the rats.3. Histological Observation of Mercury in rat tissues with amalgam fillings.Twenty female rats were used as experimental animals and ten rats were employed as controls. Experimental animals received 4 amalgamfillings. In sections from the brain and liver of the experimental animals, Hg deposits were not found, while the largest deposits of Hg were found in the kidneys. Dense accumulations of Hg were located primarily in the proximal tubule cells. Less