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Ultrastructural and immunohistochemical studies on food allergy and intestinal barrier system

食物过敏与肠道屏障系统的超微结构和免疫组织化学研究

基本信息

批准号：
09680048
负责人：
FUJITA Mamoru
金额：
$ 1.47万
依托单位：
NAKAMURA GAKUEN UNIVERSITY, FACULTY OF HOME ECONOMICS
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680048/
关键词：
food allergy ultrastructure cytochemistry immunohistochemistry intestine IgA 結腸吸収上皮細胞呼吸上皮細胞 IgA

项目摘要

In the present study, we attempted to elucidate the endocytic pathways of allergen in the jejunal and ileal absorptive cells of the neonatal and suckling rats in vivo, and whether allergen is transported across the cells. We applied macromolecule tracers to the small intestinal lumen, and examined the absorptive cells with light and electron microscopy.When tracer including ovalbumin was injected intraluminally after washed the lumen by saline as a weak barrier system model, reaction product that observed in the absorptive cells and intercellular space was increased as compared with non-treated small intestine. It is suggested that when mucus that covers the surface of the apical membrane domain decreases, non-specific macromolecules and food allergen as ovalbumin make easily an invasion into the epithelium of the jejunum and then into the lamina propria mucosae.When HRP or IgA was injected into the blood vessel, they were endocytosed into the small intestinal absorptive cells from the basolateral membrane domain, and transcytosed into the small intestinal lumen. Our results are suggested that endocytic pathways of non-specific macromolecule and IgA in the absorptive cells vary between jejunum and ileum, and also vary between suckling and adult animals. Transcytosis from basolateral plasma membrane domain to apical plasma membrane of macromolecules including IgA were occurred actively.

在本研究中，我们试图阐明过敏原的内吞途径在空肠和回肠吸收细胞的新生和哺乳大鼠在体内，过敏原是否跨细胞转运。我们将大分子示踪剂应用于小肠腔内，用光镜和电镜观察小肠吸收细胞，当用生理盐水冲洗肠腔后，向肠腔内注射包括卵清蛋白在内的示踪剂作为弱屏障系统模型时，在吸收细胞和细胞间隙中观察到的反应产物比未处理的小肠增加。提示当覆盖在顶膜区表面的粘液减少时，非特异性大分子和食物过敏原如卵清蛋白等容易侵入空肠上皮，进而进入粘膜固有层，当HRP或伊加注入血管时，它们从基底侧膜区被内吞进入小肠吸收细胞，并被胞吞到小肠腔中。这些结果表明，非特异性大分子和伊加在吸收细胞中的内吞途径在空肠和回肠之间存在差异，在哺乳动物和成年动物之间也存在差异。伊加等大分子物质从基底侧质膜域向顶侧质膜的转胞吞活动活跃。