Establishment of experimental model by using gene transfer and Leukemia Inhibitory Factor (LIF) "knock-out" mouse

基因转移和白血病抑制因子（LIF）“敲除”小鼠实验模型的建立

• 批准号: 09671502
• 负责人: FUJII Tohru
• 金额: $ 1.98万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671502/
• 关键词: Leukemia Inhibitory Factor (LIF); Wound healing; immunity in grafting; signal transduction; 動物モデル

The pituitary specific leukemia inhibitory factor (LIF) transgenic mouse revealed the significant morphological and molecular changes, which is expressed by the systemic dwarfism and the hormone producing cells in pituitary altered.The growth hormone secreting cell implantation in the adult rats resulted in the positive correlation between mandibular bone augmentation and strong LIF expression shown by in situ hybridyzation.The keloid-devived fibroblast cell culture study showed the IGF-1 receptor was specifically expressed among the tyrosine-type receptor subtypes and IGF-l was able to regulate the invasiveness of the keloid-derived fibroblast cells.This may explain that the IGF-l and its receptor pathway markedly related to the keloid mechanism and signal transduction.[IF is highly expressed in patients with psoriasis and contact dermatitis and its over-expression in local skin tissue could prolong the allogeneic skin grafting at 12, 24 and 72 hours after skin grafting on mouse back l.5x1.5 cm in between B6D2F1 and BALB/c strains. LIF Over-expressed allogeneic skin grafting resulted in the immune tolerance. This immune tolerance was regulated by LIF expressiona and and expressions of subsequent proper LIF receptor and signal transducing component namely gpl3O.Classical helper T cell expression such as Thl by IL-2 and Th2 by IL-10 were not detected. Thus this phenomena may be LIF specific and LlF may be most important regulator for the prolonged.

垂体特异性白血病抑制因子(LIF)转基因小鼠表现出明显的形态和分子变化，原位杂交结果显示，成年大鼠下颌骨生长激素分泌细胞的增加与LIF的强表达呈正相关。瘢痕疙瘩成纤维细胞培养研究表明，IGF1受体在酪氨酸型受体亚型中特异表达，IGF1受体能够调节瘢痕疙瘩成纤维细胞的侵袭性。这可能解释了IGF1及其受体通路与瘢痕疙瘩的发病机制和信号转导密切相关。皮炎及其在局部皮肤组织中的过度表达可延长同种异体皮肤移植12小时，在B6D2F1和BALB/c品系之间的小鼠背部1.5×1.5 cm处移植皮肤24小时和72小时。LIF过表达的同种异体皮肤移植导致免疫耐受。这种免疫耐受受LIFα和及随后适当的LIF受体和信号转导成分gpl30的表达调节，未检测到经典的辅助性T细胞表达，如IL-2的Th1和IL-10的Th2。因此，这种现象可能是LIF特有的，LLF可能是最重要的长期调节因素。

项目成果

Fujii Tohru,et al.: "Migration of Hydroxyatatite Onlays into the Mandible and Nasal Bone and Local Bone Turnover in Growing Rabbits." Plastic & Reconstructive Surgery.99(7). 1972-1982 (1997)

Fujii Tohru 等人：“羟基磷灰石镶嵌物迁移到下颌骨和鼻骨中以及生长中兔子的局部骨转换。”

Fujioka M,et al.: "Comparative study of inferior alveolar disturbance restoration after sagittal split osteotomy by means of bicortical versus monocortical osteosynthesis" Plast Reconstr Surg. 102. 37-41 (1998)

Fujioka M 等人：“通过双皮质与单皮质接骨术进行矢状劈开截骨术后下牙槽紊乱恢复的比较研究”Plast Reconstr Surg。

Yoshimoto H,et al.: "Increased proliferative activity of osteoblasts in congenital hemifacial Hypertrophy" Plast Reconstr Surg. 102. 1605-1610 (1998)

Yoshimoto H 等人：“先天性半面肥大中成骨细胞的增殖活性增加”Plast Reconstr Surg。

Yoshimoto H,et al.: "Overexpression of insulin-like growth factor-1(IGF-1)receptor and the invasiveness of cultured keloid fibroblasts" Am J Pathol. (in press).

Yoshimoto H 等人：“胰岛素样生长因子-1 (IGF-1) 受体的过度表达和培养的瘢痕疙瘩成纤维细胞的侵袭性”Am J Pathol。

Rashid MA,et al.: "Co-administration of Basic Fibroblast Growth Factor and Sucrose Octasulfate(Sucralfate)Facilitates the Rat Dorsal Flap Survival and Viability" Plast Reconstr Surg. (in press).

Rashid MA 等人：“碱性成纤维细胞生长因子和蔗糖八硫酸盐（硫糖铝）的共同给药促进大鼠背侧皮瓣的存活和活力”Plast Reconstr Surg。