Etiology of Cleft Palate and its gene therapy

腭裂的病因学及其基因治疗

• 批准号: 06671463
• 负责人: FUJII Tohru
• 金额: $ 1.47万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671463/
• 关键词: Parathyroid hormone related protein; Transforming growth factor beta; Anti-sense; S-oligo-DNA; Gene therapy; Cleft palate

Normal palate develops through the vertical growth, the bilateral elevation and fusion. We investigated expression of TGT beta and PTHrP in this fine process of the palate development using immunohistochemistry and in situ hybridization. Expression of both factors were recognized in the epithelium of palatal shelves and the mesenchymal condensation for bone. Concerning administration of Vitamine A induce cleft palate in pregnant rodents, reduction of PTHrP expression in embryonic facial tissue and decrease of DNA synthesis were also recognized from Vitamine A administration. However we could not show a good result of intra-amniotic administration of anti-sense S-oligo DNA for PTHrP.Although FITC-oligo was confirmed to be taken to the embryo, no remarkable change was observed. From now on, we would use organ culture, and attempt to elucidate the cleft-palate etiology.

正常腭的发育经历垂直生长、两侧抬高和融合。我们采用免疫组织化学和原位杂交技术研究了TGT β和PTHrP在腭发育过程中的表达。这两种因子的表达在腭突上皮和骨间充质凝聚中被识别。关于维生素A诱导妊娠啮齿动物腭裂的给药，也认识到维生素A给药可降低胚胎面部组织中PTHrP的表达和DNA合成。然而，我们不能显示羊膜内施用反义S-oligo DNA对PTHrP的良好结果。虽然证实FITC-oligo被带到胚胎中，但没有观察到显著变化。今后，我们将采用器官培养的方法，试图阐明腭裂的病因。

项目成果

Tsukazaki T et al: "Expression of parathyroid hormone-related protein in rat articular cartilage" Cal Tissue Int. 57. 196-200 (1995)

Tsukazaki T 等人：“大鼠关节软骨中甲状旁腺激素相关蛋白的表达”Cal Tissue Int。

Nakashima M et al: "Expression of parathyroid hormone-related peptide in human thyroid tumors." JPathol. 175. 227-236 (1995)

Nakashima M 等人：“甲状旁腺激素相关肽在人类甲状腺肿瘤中的表达。”

Tsukazaki T,Ohtsuru A,Enomoto H,Yano H,Motomura K,Ito M,Namba H,Iwasaki K,Yamashita S: "Expression of parathyroid hormone-related protein in rat articular cartilage" Cal Tissue Int. 57. 196-200 (1995)

Tsukazaki T，Ohtsuru A，Enomoto H，Yano H，Motomura K，Ito M，Namba H，Iwasaki K，Yamashita S：“大鼠关节软骨中甲状旁腺激素相关蛋白的表达”Cal Tissue Int。

Namba H et al: "Radiation-induced G1 arrest is selectively mediated by the p53-WAF1/Cip1 pathway in human thvroid cells." Cancer Res. 55. 2075-2080 (1995)

Namba H 等人：“辐射诱导的 G1 期停滞是由人类甲状腺细胞中的 p53-WAF1/Cip1 通路选择性介导的。”

Nakashima M: "Expression of parathyroid hormone-related peptide in human thyroid tumors." J.Pathol.(in press). (1995)

Nakashima M：“甲状旁腺激素相关肽在人类甲状腺肿瘤中的表达。”