Experimental Study for Circulatory and Organs Failure in Neonatal ECMO

新生儿ECMO循环及器官衰竭的实验研究

• 批准号: 09671828
• 负责人: KAMATA Shinkichi
• 金额: $ 1.79万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671828/
• 关键词: ECMO; cytokine; IL-1; IL-6; TNF; PAF; LPS; TNFアルファー; IL-1アルファー; IL-6; L-NMMA; TNアルファー

The object of this study was to determine the effects of extracorporeal membrane oxygenation (ECMO) and additional lipopolysaccharide (LPS) challenge on the degree of leukocyte activation, as measured by cytokine release in blood. ECMO was instituted in 8 puppies weighing 3 to 4 kg with flow rate of 100 ml/kg/min. After stabilization, these puppies were divided into 2 groups and studied for 210 mm. : Group Ex, consisted of 4 puppies, in which 5 mg/kg of LPS (055 : B5, Westphal type) was injected intravenously ; Group E, consisted of 4 puppies without LPS injection. White blood cell count, Systolic blood pressure and cardiac output were significantly decreased in Group Ex. Serum lactate, adrenaline, noradrenaline and dopamine were significantly increased in Group Ex. Although a significant increase of interleukin (IL)-1beta was observed in Group Ex, no significant change of blood levels of tumor necrosis factor (TNF) alpha, IL-6 and platelet-activating factor (PAF) was observed in both groups. These results indicated that the institution of ECMO may depress the blood levels of cytokines, which might cause circulatory and organs failure in neonatal ECMO.

本研究的目的是确定体外膜肺氧合（ECMO）和额外的脂多糖（LPS）挑战对白细胞活化程度的影响，通过测量血液中细胞因子的释放。在8只体重为3至4 kg的幼犬中建立ECMO，流速为100 ml/kg/min。稳定后，将这些幼犬分为2组并研究210 mm。：Ex组，由4只幼犬组成，其中静脉注射5 mg/kg LPS（055：B5，Westphal型）; E组，由4只幼犬组成，不注射LPS。Ex组的白色血细胞计数、收缩压和心输出量显著降低。Ex组血清乳酸、肾上腺素、去甲肾上腺素和多巴胺显著升高。虽然在Ex组中观察到白细胞介素（IL）-1 β显著增加，但在两组中均未观察到肿瘤坏死因子（TNF）α、IL-6和血小板活化因子（PAF）的血液水平的显著变化。结果提示，ECMO的建立可能会降低血中细胞因子的水平，从而导致新生儿ECMO时循环和器官功能衰竭。