Innervation of blood vessels in the dental pulp -a morphological study-

牙髓血管的神经支配-形态学研究-

• 批准号: 09671859
• 负责人: TABATA Shoji
• 金额: $ 2.3万
• 依托单位: KYUSHU UNIVERSITY (1998)Kagoshima University (1997)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671859/
• 关键词: dental pulp; blood vessel; nerve fiber; immunocytochemistry; SEM; neurotransmitter; 神経; 電子顕微鏡; 免疫組織化学

The innervation pattern of blood vessels in the dental pulp was investigated by scanning electron microscopy after connective tissue digestion and by transmission electron microscopy after immunohistochemical localization with polyclonal anti-PGP and anti-suhstance P antibodies. SEM specimens revealed that unmyelinated nerve fibers passed through the tunica adventitia of the blood vessels in the center of the pulp and that these fibers then entered the tunica media of the smaller arterioles. The nerve fibers divided into many collateraIs, and these terminated on the surface of smooth muscle cells and endothelial cells. The terminal fibers then reached the subodontoblastic capillary plexus and terminated on pericytes. The same results were confirmed by immunoelectron microscopy both with polyclonal anti-PGP and anti-substance P antibodies. The present study demonstrated that in the dental pulp the microvasculature as well as larger vessels were directly innervated by free nerve endings, suggesting that the local regulation of blood vessels of blood flow could take place not only at larger vessels but also at the level of the microvasculature in this tissue.

用结缔组织消化后扫描电镜和多克隆抗PGP和抗P物质抗体免疫组化定位后透射电镜观察牙髓血管神经支配模式。扫描电镜显示无髓神经纤维穿过牙髓中心血管外膜的图尼卡，然后进入小动脉的图尼卡中膜。神经纤维分裂成许多束，终止于平滑肌细胞和内皮细胞表面。终末纤维到达成牙本质细胞下毛细血管丛，终止于周细胞。用多克隆抗PGP和抗P物质抗体通过免疫电镜证实了相同的结果。本研究表明，在牙髓中的微血管以及较大的血管直接由游离神经末梢支配，这表明局部调节血管的血流不仅可以发生在较大的血管，但也在该组织中的微血管的水平。

项目成果

Shoji Tabata et al.: "Fluid flow in the dental pulp hypothesiged by a morphological study." dentistry in Japan. 34(印刷中). (1998)

Shoji Tabata 等人：“日本牙科形态学研究假设的牙髓中的流体流动”（1998 年出版）。

田畑正志 他4名: "Innervation of blood vessels in the rat incisor pulp: a scanning electron microscopic and immunoelectron microscopic study." The Anatomical Record. 251・3. 384-391 (1998)

Masashi Tabata 和其他 4 人：“大鼠门牙牙髓中血管的神经支配：扫描电子显微镜和免疫电子显微镜研究。”解剖记录 251・3（1998）。