Innervation of blood vessels in the dental pulp -a morphological study-

用多克隆抗Pgp和抗P抗体进行免疫组织化学定位后，用扫描电子显微镜和透射电子显微镜观察牙髓血管的神经分布。扫描电镜显示无髓神经纤维穿过牙髓中央的血管外膜，然后进入小动脉的中膜。神经纤维分成多条侧枝，终止于平滑肌细胞和内皮细胞表面。然后，终末纤维到达齿下母细胞毛细血管丛，并终止于周细胞。用多克隆抗Pgp和抗P物质抗体的免疫电子显微镜证实了同样的结果。本研究表明，牙髓内的微血管和大血管均直接受到游离神经末梢的支配，提示局部调节血流的血管不仅可发生在大血管上，也可发生在组织内微血管的水平上。

The innervation pattern of blood vessels in the dental pulp was investigated by scanning electron microscopy after connective tissue digestion and by transmission electron microscopy after immunohistochemical localization with polyclonal anti-PGP and anti-suhstance P antibodies. SEM specimens revealed that unmyelinated nerve fibers passed through the tunica adventitia of the blood vessels in the center of the pulp and that these fibers then entered the tunica media of the smaller arterioles. The nerve fibers divided into many collateraIs, and these terminated on the surface of smooth muscle cells and endothelial cells. The terminal fibers then reached the subodontoblastic capillary plexus and terminated on pericytes. The same results were confirmed by immunoelectron microscopy both with polyclonal anti-PGP and anti-substance P antibodies. The present study demonstrated that in the dental pulp the microvasculature as well as larger vessels were directly innervated by free nerve endings, suggesting that the local regulation of blood vessels of blood flow could take place not only at larger vessels but also at the level of the microvasculature in this tissue.