Elucidation of Central Analgesic Mechanism during Cavity Preparations -Channel Analysis of Osmosensitive Neurons within the Nucleus of the Solitary Tract-

1. Electrophysiological recordings were carried out in the whole cell current-clamp configuration on the Nuclear of the Solitary Tract （NTS） of the rat.灌注已从同位素aCSF转变为超声波或超声波解决方案，或负压力或积极压力已应用于全细胞管片内部和脉冲频率膜潜能和膜抵抗力已被记录。And SA channel blocker （GdCl 3）或Cl-channel blocker （SITS）已添加到完整和响应已被记录的更改。单一电子刺激被传送到脊柱触发核核的安全部分和与之相关的电流被记录通过NTS中的全细胞补丁管。5-HT被添加到了完美的和Evoked Currents的变化已经被测试过了。这些结果建议（1）NTS中的肌敏神经元，对相位机械变形的响应，具有拉伸激活和拉伸激活的离子通道。（2）extracellular hyperosmotically evoked cell shrinkage causes the stronger probability of the open-（SA cation channels）。（3） The extracellular hyposmotically evoked cell swelling is relevant to the SA Cly D1-イエD1 channel activity。（4）NTS的保守部分从脊柱触发核的保守部分接收到传入信号的保守部分。（5）综合体的神经发射器，它将脊柱触发核的秘密部分连接到NTS，可能是5-HT。

1. Electrophysiological recordings were carried out in the whole cell current-clamp configuration on the Nucleus of the Solitary Tract (NTS) of the rat. The perfusion was changed from isotonic aCSF to hypertonic or hypotonic solutions, or negative pressure or positive pressure were applied to the inside of whole-cell patch pipettes and impulse frequency membrane potential and membrane resistance were recorded. And SA channel blocker (GdCl3) or Cl- channel blocker (SITS) was added to the perfusate and the changes of the responses were recorded.2. Single electrical stimuli were delivered to the caudal part of the Spinal Trigeminal Nucleus and evoked currents were recorded through the whole-cell patch pipettes in the NTS. 5-HT was added to the perfusate and the changes of the evoked currents were examined.3. These results suggest that(1) The osmosensitive neurons in the NTS, respond to the phased mechanical deformation, have stretch activated and stretch inactivated ion channels.(2) The extracellular hyperosmotically evoked cell shrinkage causes the stronger probability of the open- (SA cation channels).(3) The extracellular hyposmotically evoked cell swelling is relevant to the SA ClィイD1-ィエD1 channel activity.(4) The caudal part of the NTS receive the incoming signals from the caudal part of the Spinal Trigeminal Nucleus.(5) The neurotransmitter of the synapse, that connects from the caudal part of the Spinal Trigeminal Nucleus to the NTS, is possibly 5-HT.