Elucidation of Central Analgesic Mechanism during Cavity Preparations -Channel Analysis of Osmosensitive Neurons within the Nucleus of the Solitary Tract-

腔内准备过程中中枢镇痛机制的阐明 -孤束核内渗透敏感神经元的通道分析 -

• 批准号: 09671954
• 负责人: IZAWA Shunji
• 金额: $ 1.15万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671954/
• 关键词: nucleus of the solitary tract; patch clamp method; osmosensitivity; stress induced analgesia (SIA); 中枢疼痛抑制機構; 弧束核

1. Electrophysiological recordings were carried out in the whole cell current-clamp configuration on the Nucleus of the Solitary Tract (NTS) of the rat. The perfusion was changed from isotonic aCSF to hypertonic or hypotonic solutions, or negative pressure or positive pressure were applied to the inside of whole-cell patch pipettes and impulse frequency membrane potential and membrane resistance were recorded. And SA channel blocker (GdCl3) or Cl- channel blocker (SITS) was added to the perfusate and the changes of the responses were recorded.2. Single electrical stimuli were delivered to the caudal part of the Spinal Trigeminal Nucleus and evoked currents were recorded through the whole-cell patch pipettes in the NTS. 5-HT was added to the perfusate and the changes of the evoked currents were examined.3. These results suggest that(1) The osmosensitive neurons in the NTS, respond to the phased mechanical deformation, have stretch activated and stretch inactivated ion channels.(2) The extracellular hyperosmotically evoked cell shrinkage causes the stronger probability of the open- (SA cation channels).(3) The extracellular hyposmotically evoked cell swelling is relevant to the SA ClィイD1-ィエD1 channel activity.(4) The caudal part of the NTS receive the incoming signals from the caudal part of the Spinal Trigeminal Nucleus.(5) The neurotransmitter of the synapse, that connects from the caudal part of the Spinal Trigeminal Nucleus to the NTS, is possibly 5-HT.

1.在大鼠孤束核（NTS）的全细胞电流钳结构中进行了电生理记录。将灌注从等渗aCSF改变为高渗或低渗溶液，或在全细胞贴片移液器内部施加负压或正压，并记录脉冲频率膜电位和膜电阻。并在灌流液中加入SA通道阻断剂（GdCl_3）或Cl-通道阻断剂（SITS），记录反应的变化.将单一电刺激递送至三叉神经脊束核的尾侧部分，并通过NTS中的全细胞贴片移液管记录诱发电流。在灌流液中加入5-HT，检测诱发电流的变化.这些结果表明：（1）NTS内的牵张敏感神经元对阶段性机械变形有反应，存在牵张激活和牵张失活的离子通道。(2)细胞外高血压引起的细胞收缩导致更强的开放性SA阳离子通道的可能性。(3)细胞外低渗诱发的细胞肿胀与SA Cl-β D1-β D1通道活性有关。(4)NTS的尾侧部分接收来自三叉神经脊束核尾侧部分的传入信号。(5)连接三叉神经脊束核尾侧部与孤束核的突触的神经递质可能是5-HT。