Study of Diagnostic Oncogene Analysis of Gastric Carcinoma

胃癌诊断癌基因分析研究

• 批准号: 09671288
• 负责人: SHIMIZU Tetsuro
• 金额: $ 1.86万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671288/
• 关键词: tumor suppressor gene; gastric carcinoma; chromosome 14; SSCP-PCR

OBJECTIVE : The purpose of this study was to determine the precise frequency of loss of heterozygosity (LOH) and to define the correlation between some clinicopathological factors and LOH in gastric carcinoma. It might lead to some important informations useful for study of metastasis of gastric carcinoma. MATERIALS AND METHODS : Tumor and paired normal mucosal tissues were obtained from surgically resected specimens. Genomic DNA was extracted according to the standard proteinase K digestion and phenol-chloroform extraction method. Allelic deletions on 14q32 were assessed with a PCR (polymerase Chain Reaction)-SSCP (single-strand conformation polymorphism) method using following five microsatellite markers : D14S65, D14S68, D14S62, D14S267, and D14S250. RESULTS : The average rate of informative data was 42.7% in all cases of gastric carcinomas analyzed. The highest incidence (61%) of LOH was observed at the D14S65 locus within the five loci examined. As for the other loci, LOH was recognized in 61% at Dl 4S68, 52% at D14562, 41% at D14S267, and 26% at D14S250. There was a significant correlation between the extent of LOH and both of lymphatic invasion and lymph node metastasis of gastric carcinoma. CONCLUSIONS We firstly reported that allelic deletion at 14q32 associated with lymphatic spread of gastric carcinoma. These findings might not only become a foothold for molecular studies on lymphatic spread of cancer and also lead to precise diagnosis of advanced disease in gastric cancer.

目的：本研究的目的是确定胃癌杂合性丢失（LOH）的精确频率，并确定一些临床病理因素与 LOH 之间的相关性。这可能会为胃癌转移的研究提供一些重要的信息。材料和方法：从手术切除的标本中获得肿瘤和配对的正常粘膜组织。根据标准蛋白酶K消化和酚-氯仿提取方法提取基因组DNA。使用以下五个微卫星标记通过 PCR（聚合酶链式反应）-SSCP（单链构象多态性）方法评估 14q32 上的等位基因缺失：D14S65、D14S68、D14S62、D14S267 和 D14S250。结果：所有分析的胃癌病例的平均信息数据率为42.7%。在所检查的五个基因座中，D14S65 基因座观察到 LOH 的最高发生率 (61%)。至于其他基因座，LOH 在 D1 4S68 处被识别为 61%，在 D14562 处为 52%，在 D14S267 处为 41%，在 D14S250 处为 26%。 LOH的范围与胃癌的淋巴管侵犯和淋巴结转移有显着相关性。结论我们首次报道14q32等位基因缺失与胃癌淋巴扩散相关。这些发现不仅可能成为癌症淋巴扩散的分子研究的立足点，也可能导致胃癌晚期疾病的精确诊断。