Coronary angiogenesis induced by VEGF gene: Comparison of angiogenic vessels and gene expression

在本课题中，我们发现Lac-Z可以通过腺病毒载体导入缺血心肌组织，含有腺病毒的VEGF165缓解缺血心肌，新的药物递送系统明胶水凝胶提高了基因治疗的效率，而无需使用腺病毒载体。H-9诱导30只家兔微血管缺血。缺血1小时后，进行基因传递。4周后，比较心肌功能和心肌血流量与缺血急性期的变化。在H-10中，我们在兔ASO模型中测试明胶水凝胶是否可以提高基因（VEGF）的递送效率。在H-11中，增加了补充实验并撰写了科学稿件。

In this term of the project, we revealed that Lac-Z could be introduced into ischemic myocardial tissue by using adeno-virus vector, that VEGF165 containing adeno-virus relieved the ischemic myocardium and that new drug delivery system: gelatin hydrogel enhanced the efficiency of gene therapy without using adenovirus vector. In H-9, microvascular ischemia was created in 30 rabbits. One hour after the ischemia, gene delivery was performed. Four weeks later, changes in myocardial function and myocardial blood flow were compared with those in the acute pahse of ischemia. In H-10, we tested whether efficiency of gene (VEGF) delivery can be enhanced by the use of gelatin hydrogel in rabbit ASO model. In H-11, supplemental experiments were added and scientific manuscript was written.