Coronary angiogenesis induced by VEGF gene: Comparison of angiogenic vessels and gene expression

VEGF基因诱导的冠状血管生成：血管生成血管和基因表达的比较

• 批准号: 09670756
• 负责人: MORI Hidezo
• 金额: $ 2.37万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670756/
• 关键词: VEGF; ASO; angiogenesis; synchrotron radiation; angiography; microsphere; adeno-virus; アデノヴィールス; アデノウイルス; マイクロスフェア-

In this term of the project, we revealed that Lac-Z could be introduced into ischemic myocardial tissue by using adeno-virus vector, that VEGF165 containing adeno-virus relieved the ischemic myocardium and that new drug delivery system: gelatin hydrogel enhanced the efficiency of gene therapy without using adenovirus vector. In H-9, microvascular ischemia was created in 30 rabbits. One hour after the ischemia, gene delivery was performed. Four weeks later, changes in myocardial function and myocardial blood flow were compared with those in the acute pahse of ischemia. In H-10, we tested whether efficiency of gene (VEGF) delivery can be enhanced by the use of gelatin hydrogel in rabbit ASO model. In H-11, supplemental experiments were added and scientific manuscript was written.

在本课题中，我们发现Lac-Z可以通过腺病毒载体导入缺血心肌组织，含有腺病毒的VEGF165缓解缺血心肌，新的药物递送系统明胶水凝胶提高了基因治疗的效率，而无需使用腺病毒载体。H-9诱导30只家兔微血管缺血。缺血1小时后，进行基因传递。4周后，比较心肌功能和心肌血流量与缺血急性期的变化。在H-10中，我们在兔ASO模型中测试明胶水凝胶是否可以提高基因（VEGF）的递送效率。在H-11中，增加了补充实验并撰写了科学稿件。

项目成果

S. Takeshita et al: "Endo-thelium-dependent relaxation of collateral microvessels following intramuscular gene transfer of vascular endothelial growth factor"Circulation. 98. 1261-1263 (1998)

S. Takeshita 等人：“血管内皮生长因子肌内基因转移后侧支微血管的内皮依赖性松弛”循环。

S.Takeshita, et al.: "Endothelium-dependent. Relaxation of collateral microvessels following intramuscular gene transfer of vascular endothelial growth factor."Circulation. 98. 1261-1263 (1998)

S.Takeshita 等人：“内皮依赖性。血管内皮生长因子的肌肉内基因转移后侧支微血管的松弛。”循环。

K. Ito, et al.: "A microangriographic technique using synchrotron radiation to visualize dermal circulation in vivo."Plast Reconst. Surg.. 102. 1128-1133 (1998)

K. Ito 等人：“一种使用同步加速器辐射来可视化体内真皮循环的微血管造影技术。”Plast Reconst。

Y. Tanaka, et al.: "Medical Applications of Synchrotron Radiation"Springer-Verlag. 200 (1998)

Y. Tanaka 等：“同步辐射的医学应用”Springer-Verlag。

盛英三,他: "微小血管撮影" BME. 11. 29-36 (1997)

Eizo Mori 等人：“微血管造影”BME 11. 29-36 (1997)。