课题基金 / 基金详情

Human hepatoma antigen analyzed by genetic engineering method

Human hepatoma antigen analyzed by genetic engineering method
基因工程方法分析人肝癌抗原
批准号:
09670181
负责人:
TAKAMI Tsuyoshi
金额:
$1.92万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998

项目摘要

项目成果

TAKAMI Tsuyoshi的其他基金

相似基金

相关文献

中文摘要
翻译
用哺乳动物细胞系表达载体pcDNA3.1将HLA-A_(24)cDNA导入不表达HLA-A_(24)基因的人肝癌细胞系Hep.3B2.1-7,使其细胞表面携带内源性抗原肽。HLA-A24基因克隆表达Hep.3B2.1-7的酸性电泳分析3B.A24经FPLO分级分离后,与野生型Hep.3B2.1-7和moke转染的Hep.3B2.1-7相比,在7个肽段中有1个肽段在第2(L)和第10(K)位具有HIA-A24锚定基序,而2个希斯顿H2 A,从剩余的6个组分中分离出1个HLA-A68结合肽、1个HIA-B35结合肽和2个未知肽,并对HLA-A24结合肽进行同源性分析,结果表明在数据库中没有同源蛋白,说明本研究有可能分离出一个新的HLA-A24结合肽,用基因工程方法强制表达HLA等分子,将为肿瘤抗原肽的研究开辟一条新的途径。
英文摘要
Human hepatoma cell line, Hep.3B2.1-7 that has no HLA-A24 gene expression, has been transduced of HLA-A24 cDNA by using mammalian cell line expression vector pcDNA3 .1 in order to carry the endogenous antigenic peptides on its cell surface. The acidic eluate of cloned HLA-A24 expressing Hep.3B2.1-7 (Hep.3B.A24) demonstrated seven significant peakes that were different from wild type Hep.3B2.1-7 and moke-transfected Hep.3B2.1-7 after FPLO fractionations.One out of seven peptide fraction had anchoring motief for HIA-A24 at second (L) and tenth (K) positions, while two histon H2A, one HLA-A68 binding peptide of macrophage migration inhibiting factor, one HIA-B35 binding peptide, and two unknown peptides were clarified from remaining six fractions.The homology research of HLA-A24 binding peptide obtained in present study demonstrated no homologous protein in data base.In conclusion, present study is possible to clarify a novel HLA-A24 binding peptide, and HLA.molecules enforced to express by genetic engineering method will open a new avenue for the research in tumor-antigenic peptides.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Are tumor-infiltrating macrophages derived from monocyte-lineage belonging to the myeloid-derived suppressor cell?
  • 批准号:
    21590415
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.08万
  • 财政年份:
    2009
  • 负责人:
    TAKAMI Tsuyoshi
  • 依托单位:
Clarification of the mechanism of charge, spin, and orbital and their control in thermoelectric cobalt oxides
The development of immunotherapy for human brain tumor using molecular biothechnology
  • 批准号:
    14571305
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.56万
  • 财政年份:
    2002
  • 负责人:
    TAKAMI Tsuyoshi
  • 依托单位:
国内基金
海外基金
HLA-A24和-A33限制性B、C型乙肝病毒核心蛋白T细胞表位的鉴定与评价
  • 批准号:
    30970146
  • 项目类别:
    面上项目
  • 资助金额:
    30.0万元
  • 批准年份:
    2009
  • 负责人:
    孟颂东
  • 依托单位: