Forensic applications of a new multiplex analysis on STRs using multi-fluorescently labeled primers

使用多荧光标记引物对 STR 进行新多重分析的法医应用

• 批准号: 09670436
• 负责人: YAMAMOTO Toshimichi
• 金额: $ 1.98万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670436/
• 关键词: STR; Multiplex PCR; Capillary electrophoresis; Legal medicine; Fluorescently labeled primer; Population genetics

A accurate and rapid typing system at three STR loci with fluorescently labeled primers was devised using a convenient DNA extraction kit from bloodstains, and this work was published in the Japanese Journal of Legal Medicine in 1997. In collaboration with a company, PE Applied Biosystem, we made a database of 9 STRs (D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317, D7S820) in 206 of Japanese with the commercially available kit, AmpFlSTR Profiler kit. This kit makes it possible to type 9 STRs by multiplex PCR using multi-colored fluorescent dyed primers. We calculated allele frequencies and some statistical values for personal identification and paternity testing at 9 STR loci. The result of this work was presented in the 17th International Society of Forensic Haemogenetics in Oslo in 1997, and published in Journal of Forensic Sciences in 1999. In 1998, we calculated allele frequencies in 207 of Japanese at 13 STR loci, 9 loci mentioned above and other 4 loci (D18S51, D21S11, D8 … More S1179, D16S539), with the AmpFlSTR Profiler plus kit and the Cofiler kit. Since foreigners, especially Asian populations are occasionally concerned the crimes in Japan currently, we also investigated allele frequencies at those 13 STR loci in 118 of Chinese (Beijing area), 122 of Burmese and 110 of Korean. As the result of this work, it was suggested this system of 13 STRs has also extremely high power of discrimination in each population, and each population has a few STR loci significantly different from Japanese in pairwise comparison of allele distribution at each loci. Furthermore, various tests for Hardy-Weinberg equilibrium were evaluated using Japanese allele frequencies at 9 STR loci, and the results were summarized and published in DNA Polymorphisms.We applied the AmpFlSTR Profiler kit to a paternity testing, so-called an isolated exclusion case, in which the biological fatherfood was excluded at only one locus (D3S1358 in this case) in 15 loci examined. It was confirmed that the exclusive allele at D3S1358 was derived from paternal mutantion using MVR-PCR at D1S8 and D7S21. This work was presented in the 82nd annual meeting of Japanese Society of Legal Medicine. We also applied this system to the personal identification of decomposed cadavers using their toothbrushes kept in houses as reference samples. Less

利用方便的血痕DNA提取试剂盒，设计了一种在三个STR基因座上使用荧光标记引物的准确和快速分型系统，这项工作于1997年发表在日本法律的医学杂志上。我们与PE Applied Biosystem公司合作，使用市售的AmpFlSTR Profiler试剂盒，在206名日本人中建立了9个STR（D3 S1358，vWA，FGA，TH 01，TPOX，CSF 1 PO，D5 S818，D13 S317，D 7S 820）的数据库。该试剂盒可以使用多色荧光染色引物通过多重PCR对9个STR进行分型。计算了9个STR基因座的等位基因频率，并对个体识别和亲权鉴定进行了统计分析。这项工作的结果于1997年在奥斯陆举行的第17届国际法医血液发生学学会上发表，并于1999年发表在《法医科学杂志》上。1998年我们对207名日本人在13个STR基因座、上述9个STR基因座和其他4个STR基因座（D18 S51、D21 S11、D8 ...更多信息 S1179，D16 S539），AmpFlSTR Profiler plus试剂盒和Cofiler试剂盒。鉴于目前外国人，特别是亚洲人对日本犯罪的关注，我们还调查了118名中国人（北京地区）、122名缅甸人和110名韩国人的13个STR基因座的等位基因频率。作为这项工作的结果，它被认为是这个系统的13个STR在每个群体中也有极高的区分力，每个群体有几个STR基因座显着不同的等位基因分布在每个基因座的两两比较。此外，使用9个STR基因座的日本等位基因频率对Hardy-Weinberg平衡进行了各种测试，并将结果汇总并发表在DNA Polymorphisms上。我们将AmpFlSTR Profiler试剂盒应用于亲子鉴定，即所谓的孤立排除案例，其中生物学父亲食物仅在15个基因座中的一个基因座（此例中为D3 S1358）被排除。MVR-PCR结果表明，D3 S1358位点的等位基因是由D1 S8和D 7S 21位点的父本突变引起的。这项工作在日本法律的医学学会第82届年会上发表。我们也将这个系统应用到个人身份的腐烂尸体使用他们的牙刷保持在房子作为参考样本。少

项目成果

Yamamoto T.: "Allele distribution at nine STR loci-D3S1358, vWA,FGA,TH01, TPOX,CSF1PO,D5S818, D13S317 and D7S820-in the Japanese population by multiplex PCR and capillary electrophoresis." Journal of Forensic Sciences. 44(1). 167-170 (1999)

Yamamoto T.：“通过多重 PCR 和毛细管电泳分析日本人群中九个 STR 位点（D3S1358、vWA、FGA、TH01、TPOX、CSF1PO、D5S818、D13S317 和 D7S820）的等位基因分布。”

Yamamoto,Toshimichi: "Polymorphism analysis of nine STR loci in Japanese using multiplex PCR and capillary electrophoresis." Progress in Forensic Genetics. 7. 390-391 (1998)

Yamamoto, Toshimichi：“使用多重 PCR 和毛细管电泳对日本人的 9 个 STR 基因座进行多态性分析。”

Yamamoto T.: "A rapid typing system at three STR loci from bloodstains using a simple DNA extraction kit and capillary electrophoresis." Japanese Journal of Legal Medicine. 51(5). 396-400 (1997)

Yamamoto T.：“使用简单的 DNA 提取试剂盒和毛细管电泳对血迹中的三个 STR 位点进行快速分型系统。”

Yamamoto,Toshimichi: "A rapid typing system at three STR loci from bloodstains using a simple DNA extraction kit and capillary electrophoresis." Japanese Journal of Legal Medicine. 51(5). 396-400 (1997)

Yamamoto, Toshimichi：“使用简单的 DNA 提取试剂盒和毛细管电泳对血迹中的三个 STR 位点进行快速分型系统。”

Yamamoto T.: "Polymorphism analysis of nine STR loci in Japnaese using multiplex PCR and capillart electrophoresis." Progress in Forensic Genetics. 7. 390-391 (1998)

Yamamoto T.：“使用多重 PCR 和毛细管电泳对日本人的 9 个 STR 基因座进行多态性分析。”