Production of transgenic plants and physiological characteritics of the plants.

以粳稻（Oryza sativa cv. Notohikari）已经通过使用碳化硅晶须（SCW）或硼酸铝晶须（ABW）将DNA递送到从成熟种子分离的胚的盾叶组织中来实现。将质粒DNA与SCW或ABW混合，以GUS报告基因在组织中的瞬时表达为基础，确定了SCW或ABW介导DNA转移的一些参数。在此条件下，将抗双丙氨磷的bar基因和GUS基因一起导入到水稻组织中，获得了双丙氨磷抗性愈伤组织。从它们再生出转基因水稻植株。GUS组织化学分析和PCR分析证实了GUS基因和bar基因的整合。Southern杂交分析也证实了bar基因的整合。这些结果表明，一个简单和廉价的DNA传递程序通过ABW可以是有用的，以产生转基因水稻植株。

A transformation of a japonica rice (Oryza sativa cv. Notohikari) has been accomplished by the use of silicon carbide whiskes (SCW) or aluminum borate whiskers (ABW) to deliver DNA into the scutellar tissues of embryos isolated from mature seeds. DNA delivery was mediated by mixing in the presence of plasmid DNA and SCW or ABW.Some parameters of DNA delivery employing SCW or ABW were determined in the tissues based on the transient expression of b-glucronidase (GUS) reporter gene. The optimum condition was established as the mixing of 290 ul buffer containing 25 ug DNA, 20 tissues and 2 mg of SCW or 64 mg ABW for 30 min. Under these conditions, we tried to introduce bar gene whose product confers resistance to the bialaphos into the tissues, together with GUS gene, and obtained the bialaphos resistance calli. Transgenic rice plants were regenerated from them. The integration of GUS gene and bar gene were confirmed by GUS histochemical assay and PCR analysis, respectively. Southern hybridization analysis alsoconfirmed the integration of bar gene. These results suggest that a simple and inexpensive DNA delivery procedure via ABW can be useful to produce transgenic rice plants.