Production of transgenic plants and physiological characteritics of the plants.

粳稻（Oryza sativa cv.）的转化。Notohikari)是通过使用碳化硅晶须（SCW）或硼酸铝晶须（ABW）将DNA传递到从成熟种子中分离出来的胚胎的胸膜组织中来实现的。在质粒DNA和SCW或ABW存在的情况下，通过混合介导DNA传递。根据b-葡萄糖苷酶（GUS）报告基因的瞬时表达，确定了SCW或ABW在组织中传递DNA的一些参数。最佳条件为290 ul含25 ug DNA的缓冲液，20个组织和2 mg SCW或64 mg ABW混合30 min。在此条件下，我们尝试将具有抗性的bar基因与GUS基因一起导入组织，获得了抗双磷愈伤组织。从它们身上再生出转基因水稻植株。GUS基因和bar基因的整合分别通过GUS组织化学和PCR分析得到证实。南方杂交分析也证实了bar基因的整合。这些结果表明，通过ABW的一种简单而廉价的DNA传递程序可用于生产转基因水稻。

A transformation of a japonica rice (Oryza sativa cv. Notohikari) has been accomplished by the use of silicon carbide whiskes (SCW) or aluminum borate whiskers (ABW) to deliver DNA into the scutellar tissues of embryos isolated from mature seeds. DNA delivery was mediated by mixing in the presence of plasmid DNA and SCW or ABW.Some parameters of DNA delivery employing SCW or ABW were determined in the tissues based on the transient expression of b-glucronidase (GUS) reporter gene. The optimum condition was established as the mixing of 290 ul buffer containing 25 ug DNA, 20 tissues and 2 mg of SCW or 64 mg ABW for 30 min. Under these conditions, we tried to introduce bar gene whose product confers resistance to the bialaphos into the tissues, together with GUS gene, and obtained the bialaphos resistance calli. Transgenic rice plants were regenerated from them. The integration of GUS gene and bar gene were confirmed by GUS histochemical assay and PCR analysis, respectively. Southern hybridization analysis alsoconfirmed the integration of bar gene. These results suggest that a simple and inexpensive DNA delivery procedure via ABW can be useful to produce transgenic rice plants.