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STUDIES ON PREPARATIVE SCALE SEPARATION AND PURIFICATION OF RETICULOENDOTHELIAL SYSTEM ACTIVATION POLYSACCHARIDE FROM SNACHI-GINSENG

西洋参网状内皮系统激活多糖的制备规模分离与纯化研究

基本信息

批准号：
60870078
负责人：
TANAKA Osamu
金额：
$ 5.76万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

The preparative scale separation and purification of a reticuloendothelial system activating polysaccharide from Sanchi-Ginseng (root of Panax notoginseng), the well-known Chinese traditional medicine, was investigated. By means of gel permeation high performance liquid chromatogaraphy on large columns of a variety of synthetic polymers (polyvinyl gel and porous polymer gel etc.), an active fraction (2A-1) was obtained. Treatment of 2A-1 with amyloglucosidase gave glucose and an unhydrolyzed arabinogalactan named sanchinan-A(SA), demonstrating that 2A-1 was a mixture of a glucan and SA with the similar molecular weight(1,600,000) to each other. It was further revealed that the activity of 2A-1 must be represented by SA. The structure of SA was established to be composed of a <beta> -D-(1--3)-linked galactopyranosyl-backbone possessing branching points at position 0-6 to which [mainly <alpha> -L-arabinofuranosyl and partly <beta> -D-galactopyranosyl] -(1--6)- <beta> -D-galactopyranosyl-(1--3)- <beta> -D-galactopyranosyl side chain are attached on average to two of three galactosyl units. SA contains a small amount of protein (3.27%). By the similar procedures as above, active polysaccharides were isolated from Ginseng and Chikusetsu-Ninjin (rhizome of P. japonicus). These were proved to be different in molecular weight and sugar component from SA.In order to develope the efficient separation of arabinogalactan such as SA from glucan, high-performance liquid chromatography of borate complexes of a variety of glycosides on a basic ion-exchange column was investigated. It was revealed that this procedure is effective for the separation of isomeric glycosides, containing arabinofuranosyl, arabinopyranosyl or galactopyranosyl units from those containing xylopyranosyl and glucopyranosyl units.

本文研究了从中药三七中提取的网状内皮系统激活多糖的制备级分离纯化工艺。采用凝胶渗透高效液相色谱法，在大柱上对多种合成聚合物（聚乙烯基凝胶和多孔聚合物凝胶等），得到活性级分（2A-1）。用淀粉葡糖苷酶处理2A-1得到葡萄糖和未水解的阿拉伯半乳聚糖，命名为sanchinan-A（SA），表明2A-1是具有彼此相似的分子量（1，600，000）的葡聚糖和SA的混合物。进一步揭示了2A-1的活性必须用SA表示。SA的结构被确定为由<beta>在0-6位具有分支点的-D-（1- 3）-连接的吡喃半乳糖基骨架组成，[主要是<alpha>-L-阿拉伯呋喃糖基和部分<beta>-D-吡喃半乳糖基] -（1- 6）-<beta>β-D-吡喃半乳糖基-（1- 3）-<beta>β-D-吡喃半乳糖基侧链平均连接到三个半乳糖基单元中的两个。SA含有少量的蛋白质（3.27%）。采用与上述相似的方法，从人参和人参的根茎中分离出活性多糖。这些被证明是不同的分子量和糖组分从SA。为了开发阿拉伯半乳聚糖，如SA从葡聚糖的有效分离，高效液相色谱的硼酸盐络合物的各种糖苷的碱性离子交换柱进行了研究。结果表明，这种方法是有效的分离异构体糖苷，含有阿拉伯呋喃糖，阿拉伯吡喃糖或半乳糖吡喃糖单元从那些含有木吡喃糖和葡萄糖吡喃糖单元。