Inheritance of malonylated anthocyanin production and distribution of the pigment in fruit of strawberry cultivars

草莓品种果实中丙二酸花青素生成及色素分布的遗传

• 批准号: 09660032
• 负责人: YOSHIDA Yuichi
• 金额: $ 2.18万
• 依托单位: OKAYAMA UNIVERSITY (1998-1999)Kagawa University (1997)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660032/
• 关键词: strawberry; anthocyanin; fruit color; inheritance; Pelargonidin 3-malonylglucoside; cultivar difference; strawberry; anthocyanin; fruit color; inheritence

In 'Nyoho', the concentration of total anthocyanins increased linearly during fruit coloring, however the hue angle of fruit skin did not change from 41 days after anthesis. The composition of Pelargonidin 3-malonylglucoside (Pdg-3MG) was ca.20% of total anthocyanins throughout fruit coloring. Among 20 cultivars examined, Pdg-3MG occurred in 11 and did not in the rest. Although the concentration of total anthocyanins varied much among cultivars, there were no significant difference between two groups of cultivars producing Pdg-3MG and lacking it. The occurrence or absence may not affect color development of strawberry fruits.Selfed progeny of Pgd-3MG producing 'Nyoho' segregated into 84 producing and 25 lacking plants. However, when analyzed more precisely, trace level (less than 1% of anthocyanins) of Pdg-3MG could be detected. Progenies of 'Nyoho' and 'Darselect' segregated into 64 : 8 : 11 and 35 : 10 : 3 for high (>4.4%) : trace (<0.7%) : non in Pdg-3MG composition, respectively. It may indicate that the production of malonylated anthocyanin in strawberry is controlled by two dominant genes those produce much Pgd-3MG (Pmg-1) and trace level (Pmg-2).The ratio in number of cultivars those lacking Pgd-3MG to producing, was higher in Japanese cultivars than in foreign cultivars. Within Japanese cultivars, many of those released from Kurume Branch of NIVOT, such as 'Harunoka', are lacking Pgd-3MG. There could not be found any relationships between the occurrence of Pgd-3MG and practical fruit characteristics, such as sugar concentration, composition or color of fruit.

在‘Nyoho’中，总花青素浓度在果实着色过程中线性增加，但果皮的色调角从开花后41天开始没有变化。天竺葵素 3-丙二酰葡萄糖苷 (Pdg-3MG) 的组成约占整个果实着色过程中总花青素的 20%。在检查的 20 个品种中，11 个品种出现 Pdg-3MG，其余品种未出现。尽管不同品种间总花色苷含量差异较大，但产生Pdg-3MG和缺乏Pdg-3MG的两组品种之间无显着差异。产生“Nyoho”的Pgd-3MG的自交后代分为84株产生株和25株缺失株。然而，当进行更精确的分析时，可以检测到痕量（低于 1% 的花青素）的 Pdg-3MG。 'Nyoho' 和'Darselect' 的后代在 Pdg-3MG 组合物中分别分离为 64:8:11 和 35:10:3 的高 (>4.4%) : 痕量 (<0.7%) : 无。这可能表明草莓中丙二酰化花青素的产生是由产生大量Pgd-3MG(Pmg-1)和微量(Pmg-2)的两个显性基因控制的。日本品种中缺乏Pgd-3MG的品种数量所占的比例高于国外品种。在日本品种中，许多从 NIVOT 久留米分公司发布的品种（例如“Harunoka”）都缺乏 Pgd-3MG。未发现 Pgd-3MG 的发生与实际水果特性（如糖浓度、水果成分或颜色）之间存在任何关系。

项目成果

Yoshida, Y. et al.: "Inheritance of Pelargonidin malonylglucoside synthesis in strawberry"Acta Horticulturae. (in press).

Yoshida, Y. 等人：“草莓中天竺葵素丙二酰葡萄糖苷合成的继承”园艺学报。

吉田裕一ほか: "イチゴの果色とPelargonidin 3-malonyl-glucoside生成能の品種間差異"園芸学会雑誌. 66(別2). 456-457 (1997)

Yuichi Yoshida 等人：“草莓果实颜色和天竺葵素 3-丙二酸单糖苷生产能力的差异”，日本园艺学会杂志 66（第 2 部分）（1997 年）。

Yoshida, Y. et al.: "Differences in color and Pelargonidin 3-malonyl-glucoside synthesis in strawberry cultivars"Jour. Japan Soc. Hort. Sci.. 66(Suppl. 2). 456-457 (1997)

Yoshida, Y. 等人：“草莓品种中颜色和天竺葵素 3-丙二酰基-葡萄糖苷合成的差异”Jour。

吉田 裕一 ほか: "イチゴの果色と Pelargonidin 3-malonyl-glucoside 生成能の品種間差異" 園芸学会雑誌. 66・別2. 456-457 (1997)

Yuichi Yoshida 等人：“草莓果实颜色和天竺葵素 3-丙二酰葡萄糖苷生产能力的品种差异”日本园艺学会杂志 66，第 2 部分。456-457（1997 年）。

Yoshida,Y.et al.: "Inheritence of Pelargonidin malonylglucoside Synthesis in Strawberry" Acta Horticulturae. (in press).

Yoshida，Y.et al.：“草莓中天竺葵素丙二酰葡萄糖苷合成的继承”园艺学报。