Analysis of species specificity in endoparasitoids

内寄生蜂的物种特异性分析

• 批准号: 09660047
• 负责人: TANAKA Toshiharu
• 金额: $ 1.66万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660047/
• 关键词: host specificity; endoparasitoid; stage specificity; Cotesia kariyai; Cotesia ruficrus; Pseudaletia separata; Pseudaletia separata; カリヤコマユバチ; イネヨトウコマユバチ; ポリドナウイルス

Larval endoparasitoids have to regulate the host in the physiological condition to acquire the suitable state for the development of their eggs and larvae. Between two different parasitoids being able to attack the same host species (Pseudaletia separata), one parasitoid, Cotesia ruficrus is restricted to parasitize the young stadium of the host, while another parasitoid, Cotesia kariyai is able to parasitize successfully through all stadium. Reciprocal exchanging of the polydnavirus and the venom of each parasitoid produced the host regulation according to the characteristics of the polydnavirus. Each genomic DNA segments hybridized with the reciprocal genornic DNA segments of PDV.Furthermore, we reported the cloning and characterization of Cotesia kariyai polydnavirus (CkPV) genes expressed in host Pseudaletia separata testes. We isolated clones encoding CkPV mRNAs from a cDNA library of host testes. We found two motif sequences of the RNase T_2 family in one of CkPV gene expressed in host testes. The CkPV RNase T_2 fragment was hybridized to more than one viral genome segments. By northern blot analysis using cloned CkPV RNase T_2 gene as a probe, we showed the 3.1 kbp and 2.3 kbp mRNAs were detected 4-12h after parasitization, which Was before the appearance of cells with abnormal chromatin in the host testes.

幼虫类内寄生虫需要调节寄主的生理状态，使寄主的卵和幼虫发育到适宜的状态。在两种不同的寄生蜂能够攻击同一种寄主（分离伪蜂）之间，一种寄生蜂rutesia ruficrus被限制寄生在寄主的幼运动场，而另一种寄生蜂Cotesia kariyai能够成功寄生在所有运动场。多dna病毒与各种拟寄生物的毒液相互交换，根据多dna病毒的特点产生寄主调控。每个基因组DNA片段与PDV的互反基因组DNA片段杂交。此外，我们还报道了在宿主分离假假蝇中表达的凯里虫多dna病毒（Cotesia kariyai polydnavvirus, CkPV）基因的克隆和鉴定。我们从宿主睾丸的cDNA文库中分离出编码CkPV mrna的克隆。我们在宿主睾丸表达的CkPV基因中发现了两个RNase T_2家族基序序列。将CkPV RNase T_2片段与多个病毒基因组片段杂交。以克隆的CkPV RNase T_2基因为探针，通过northern blot分析，我们发现，在寄主睾丸出现染色质异常细胞之前，在寄主睾丸中寄生4-12h后检测到3.1 kbp和2.3 kbp的mrna。

项目成果

田中 利治: "ポリドナウイルス-幼虫寄生バチの共生者" 植物防疫. 52. 499-504 (1998)

Toshiharu Tanaka：“多DNA病毒 - 幼虫寄生黄蜂的共生体”植物保护 52. 499-504 (1998)。

Toshiharu Tanaka: "Polydnavirus-symbiont of larval endoparasitoid" Shokubutu-Boeki. 52(in Japanese). 499-504 (1998)

Toshiharu Tanaka：“多DNA病毒-内寄生幼虫的共生体”Shokubutu-Boeki。

田中 利治: "ポリドナウイルス一幼虫寄生バチの共生者" 植物防疫. 52. 499-504 (1998)

Toshiharu Tanaka：“多DNA病毒 - 幼虫寄生蜂的共生体”植物保护 52. 499-504 (1998)。