Analysis of regulational mechanism of growth and development of testis

睾丸生长发育调控机制分析

• 批准号: 11460020
• 负责人: TANAKA Toshiharu
• 金额: $ 2.94万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460020/
• 关键词: Endoparasicoid; Polydnavirus; chlorofluazuron; vas deference; testis; 寄生蜂; RNaseT2

( 1 ) Parasitic castration of host, Pseudaletia separata, was caused by polydnavirus (CkPDV) and venom of Cotesia kariyai. CkPDV was expressed in testicular cells damaged like apoptosis. CkPDV genes expressed in damaged testicular cells contained sequence of two active sites of RNase T2. Damaged cells was well stained with anti-RNase T2 antibody which was produced against protein expressed in bacteria. RNase T2 family was related with self-incompatibility. However, it was unclear how RNase T2 gene was relation to decomposed testicular cells after meiotic block of cell cycling. Recently, big oil droplets were observed in fat body cells of parasitized host after parasitization. It seemed to be caused by destruction of tubulin in the cells. It was suggested that CkPDV affected the formation of tublin in the cells, so that CkPDV disturbed the formation of the spindle in mitosis or meiosis of the testis.( 2 ) Chlorfluazuron depressed the number of progeny when chorfluazuron was applied on Spodoptera litura larvae at previous last larval stage and delay the sperm movement to vas deference. It was examined whether same effects was duplicated or not, in Pseudaletia separata, belonged to same Noctuidae family. Chlorfluazuron did not affect the delation of movement of sperm, although the growth of testis was depressed in volume.

(1)寄生阉割寄主是由多聚核糖核酸病毒(CkPDV)和卡氏梭子虫毒液引起的。CkPDV在受损的睾丸细胞中呈凋亡样表达。在受损睾丸细胞中表达的CkPDV基因含有两个RNaseT2活性位点序列。抗核糖核酸酶T2抗体对受损细胞有良好的染色效果。RNaseT2家族与自交不亲和相关。然而，RNaseT2基因与细胞减数分裂阻断后分解的睾丸细胞之间的关系尚不清楚。最近，在寄生寄主的脂肪体细胞中观察到了大的油滴。这似乎是由于细胞内微管蛋白的破坏所致。提示CkPDV影响细胞内微管蛋白的形成，从而干扰了精巢有丝分裂或减数分裂纺锤体的形成。(2)在斜纹夜蛾幼虫末期幼虫上施敌百隆，可抑制子代数量，延缓精子向精管的移动。对同属夜蛾科的粘虫是否具有相同的作用进行了检验。尽管睾丸体积生长受到抑制，但氯氟脲对精子运动的延迟没有影响。

项目成果

Nakamatsu, Y., Y.Gyotoku, T.Tanaka: "The parasitoid Cotesia kariyai (Ck) regulates the growth and metabolic efficiency of Pseudaletia separata larvae by venom and Ck polydnavirus"J. Insect Physiol.. 47. 573-584 (2001)

Nakamatsu, Y., Y.Gyotoku, T.Tanaka：“寄生蜂 Cotesia kariyai (Ck) 通过毒液和 Ck 多DNA病毒调节 Pseudaletia separata 幼虫的生长和代谢效率”J.

Takada, Y., S. Kawamura, T. Tanaka: "Host preference of Trichogramma dendrolimi(Hymenoptera Trichogrammatidae) on its native host, Mamestra brassicae (Lepidoptera : Noctuidae) after 12 continuous on a factitious host"Appl. Entomol. Zool. 36. 213-218 (2001

Takada, Y., S. Kawamura, T. Tanaka：“在连续 12 次在人造宿主上后，松毛虫赤眼蜂（膜翅目赤眼蜂科）对其天然寄主马梅斯特拉·芸苔科（鳞翅目：夜蛾科）的寄主偏好”。

田中 利治: "昆虫から学ぶ生きる知恵 第15回「大学と科学」公開シンポジウム組織委員会編"クバプロ. 174 (2001)

Toshiharu Tanaka：“从昆虫中学习生活智慧：第 15 届‘大学与科学’公共研讨会组织委员会编辑”，KubaPro 174 (2001)。

I Nakamatsu. Y., Y. Gyotoku, T. Tanaka,: "The parasitoid Cotesia kariyai (Ck) regulates the growth and metabolic efficiency of Pseudaletia separata larval by venom and Ck polydnavirus"J. Insect Physiol.. 47. 573-584 (2001)

我是中松。

Takada, Y.; S. Kawamura and T. Tanaka: "Biological characteristics : growth and development of the egg parasitoid Trichogramma dendrolimi (Hymenoptera : Trichogrammatidae) on the cabbage armyworm Mametra brassicae (Lepidoptera : Noctuidae)."Appl. Entomol.

高田，Y.；