Inhibitory Effects of Amino-carbonyl Reactions Occurred between bovine beta-lactoglobulin and Lactose on the Oral Tolerance-inducing Activity of the Native Protein Antigen.
牛β-乳球蛋白和乳糖之间发生的氨基-羰基反应对天然蛋白抗原的口服耐受诱导活性的抑制作用。
基本信息
- 批准号:09660130
- 负责人:
- 金额:$ 2.11万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In the present study, we examined whether oral tolerance directed against bovine beta- lactoglobulin (beta -Lg), one of the most potent allergens in cow's milk, was induced by feeding of cow's milk with or without heat treatments at the applied temperature ranging from 60゚C to 90 ゚C for a period of 10-300 min. Groups of female C3H/HeN mice, a higher responder to (3 -Lg, were orally given with about 3 mI/day/head of the milk or heated milk during 2-62 days prior to the immunization with beta-Lg. The lymph node cells were then tested for their in vitro antigen-specific proliferative response by measuring ^3H-thymidine uptake (T cell proliferation assay) and their sera were also investigated for the binding to beta -Lg by an enzyme-linked immunosorbent assay (ELISA). The feeding of raw milk for 8 days was usually found to lower the both levels of antibody and T cell responses against beta-Lg to less than 1/10 as compared with the control mice, and the unresponsiveness state was held almost constant even after the longer feeding of the milk. The oral tolerance, however, could not be well induced by feeding of the heated milk. Such inhibition of the tolerance was gradually enhanced with increasing the applied temperature, time and the feeding period.Next, we studied why the poor tolerance was induced by feeding with the heated milk in mice. Five groups of mice were orally administrated with PBS (control), beta-Lg, beta-Lg plus lactose, heated beta-Lg and heated beta-Lg plus lactose, respectively, and compared the magnitude of immunological tolerance observed in each group of mice. The significant inhibition of oral tolerance specific for both beta-Lg and heated beta-Lg plus lactose was shown in only the group given with heated beta-Lg plus lactose, suggesting that amino-carbonyl (Maillard) reactions occurred between beta- Lg and lactose may lower the oral tolerance-inducing activity of the native protein antigen.
在本研究中,我们检测了是否口服耐受针对牛β-乳球蛋白,(β-Lg)是牛奶中最有效的过敏原之一,通过在60 ℃至90 ℃的应用温度范围内加热处理或不加热处理牛奶10-300分钟来诱导。(3 -Lg,在用β-Lg免疫前2-62天,每天口服约3 mI/头的牛奶或加热牛奶,然后通过测量^3H-胸苷摄取(T细胞增殖试验)检测淋巴结细胞的体外抗原特异性增殖反应,并通过一种酶-免疫吸附试验(ELISA)。通常发现,与对照小鼠相比,喂食生牛奶8天,针对β-Lg的抗体和T细胞应答水平均降低至1/10以下,并且即使在较长时间喂食牛奶后,无应答状态也几乎保持不变。但热牛奶不能很好地诱导小鼠的口服耐受。这种耐受性的抑制作用随着温度、时间和喂养时间的增加而逐渐增强。将5组小鼠分别口服给予PBS(对照)、β-Lg、β-Lg加乳糖、加热的β-Lg和加热的β-Lg加乳糖,并比较在各组小鼠中观察到的免疫耐受的程度。仅在给予加热的β-Lg加乳糖的组中显示出对β-Lg和加热的β-Lg加乳糖两者特异性的口服耐受性的显著抑制,这表明β- Lg和乳糖之间发生的氨基-羰基(美拉德)反应可能降低天然蛋白抗原的口服耐受性诱导活性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
榎本 淳 他: "牛乳および加熱乳の自由摂取により誘導されるβ-ラクトグロブリン(β-Lg)に特異的な免疫抑制効果" 日本農芸化学会誌. 73. 146-146 (1999)
Jun Enomoto等:“随意摄入牛奶和加热牛奶引起的β-乳球蛋白(β-Lg)特异性免疫抑制作用”日本农业化学学会杂志73. 146-146(1999)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
榎本 淳他: "牛乳および加熱乳の自由摂取により誘導されるβ-ラクトグロブリン(β-Lg)に特異的な免疫抑制効果" 日本農芸化学会誌. 73. 146-146 (1999)
Jun Enomoto 等人:“随意摄入牛奶和加热牛奶引起的 β-乳球蛋白 (β-Lg) 特异性免疫抑制效应”日本农业化学学会杂志 73. 146-146 (1999)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A.Enomoto et al.: "Immunosuppressive Effects against Bovine beta-lactoglobulin Induced by Oral Administration of Cow's Milk with or without Heat Treatments." Nippon Nogeikagaku Kaishi(in Japanese). 73. 146 (1999)
A.Enomoto 等人:“口服经过或不经过热处理的牛奶诱导的对牛 β-乳球蛋白的免疫抑制作用”。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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ENOMOTO Atsushi其他文献
ENOMOTO Atsushi的其他文献
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A milk peptide fraction for preventing the allergies and autoimmune diseases
用于预防过敏和自身免疫性疾病的牛奶肽成分
- 批准号:
26660105 - 财政年份:2014
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Immune regulation systems in milk
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23658108 - 财政年份:2011
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$ 2.11万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Molecular mechanisms for neurogenesis and psychiatric dysfunction in postnatal and adult periods
产后和成年期神经发生和精神功能障碍的分子机制
- 批准号:
21689013 - 财政年份:2009
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$ 2.11万 - 项目类别:
Grant-in-Aid for Young Scientists (A)
Radiosensitization by targeting a novel MAP3K negative regulator STK38
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- 批准号:
20591491 - 财政年份:2008
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Grant-in-Aid for Scientific Research (C)
Roles of the novel Akt substrate Girdin and its family of proteins in development and tumor protression
新型 Akt 底物 Girdin 及其蛋白家族在发育和肿瘤进展中的作用
- 批准号:
18689904 - 财政年份:2006
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Grant-in-Aid for Young Scientists (A)
Immunosuppressive effects induced by oral administration of peptide fragments derived from bovine β -lactoglobulin
口服牛β-乳球蛋白肽片段诱导的免疫抑制作用
- 批准号:
13660117 - 财政年份:2001
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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