Study on quantification of opportunistically infectious microorganisms in sewage and treated sewage and safety assessment of wastewater reclamation.
污水及处理后污水中机会性传染性微生物的定量研究及废水回收的安全评估。
基本信息
- 批准号:09650605
- 负责人:
- 金额:$ 1.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Water quality of reclaimed wastewater is required new standard on health related microorganisms. Quantity of treated wastewater reused has been increased especially in developed urban arias. We water quality scientists and engineers have almost only been interested in the pathogens. Under the resent situation above mentioned, however, another important attention should be taken for opportunistic infection, because an unspecified number of people including handicapped persons in health are eventually exposed to water born opportunistically infectious microorganisms.In this study, the researcher developed new detection methods of Legionellapneumophila, which is an important water born opportunistic-infection bacteria and causes legionnaire disease, in sewage. Polymerase chain reaction (PCR) and DNA-DNA hybridization methods were employed to detect L.pneumophila and PCR method was improved to be applied to quantitative detection of L.pneumophila.The results from PCR-DNA hybridization test … More show the possibility that L.pneumophila survives through secondary sewage treatment and disinfection process in which conventional selective media for L.pneumophila are not available. The experimental data of PCR-MPN for L.pneumophila shows good correlation to the enumeration data obtained by fluorescence stained direct count of this bacteria.The researcher also challenged to establish a molecular biological method for the detection of L.pneumophila viable in sewage. Te bacterial colonies grown on a nylon membrane filter put on a selective BCYE-alpha agar plate that contains four antibiotics which do not restrain the growth of L.pneumophila were processedthrough the colony-hybridization previously developed. This new colony-hyblidization with a L.pneumophila-specific mip DNA probe showed that the L.pneumophila colonies were distinguished from other non-Legionella coloniesdeveloped om the memblane filter on the agar medium. Therefore, viable (colony-forming) L.pneumophila in sewage can be detected selectively and quantativelyby using the new colony hyblidization method. Less
对中水水质提出了新的卫生微生物标准要求。特别是在发达的城市地区,处理后的污水回用数量有所增加。我们水质科学家和工程师几乎只对病原体感兴趣。然而,在上述情况下,另一个重要的关注点是机会感染,因为包括健康障碍者在内的数量不详的人最终会接触到水生机会感染微生物。在本研究中,研究人员开发了一种新的检测方法,军团菌是一种重要的水生机会感染细菌,可引起军团病。采用聚合酶链式反应和DNADNA杂交法对嗜肺性乳杆菌进行检测,并对聚合酶链式反应方法进行改进,将其应用于嗜肺乳杆菌的定量检测。聚合酶链式反应-DNA杂交试验…更多的证据表明,嗜肺乳杆菌可能通过二级污水处理和消毒工艺存活下来,而在这些过程中,嗜肺乳杆菌的常规选择性培养基是不可用的。嗜肺乳杆菌的PCR-MPN实验数据与荧光染色直接计数法获得的计数数据具有良好的相关性,研究人员还提出了建立检测污水中活菌的分子生物学方法。将生长在尼龙膜过滤器上的细菌菌落放在含有四种不抑制嗜肺乳杆菌生长的抗生素的选择性BCYE-α琼脂平板上,通过先前开发的菌落杂交来处理。用嗜肺乳杆菌特异的MIP DNA探针进行的这种新的菌落杂交表明,嗜肺乳杆菌菌落有别于其他非军团菌菌落,这些非军团菌是在琼脂滤膜上形成的。因此,利用新的菌落杂化方法可以选择性、定量地检测污水中的活菌(菌落形成)嗜肺乳杆菌。较少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.Ishigami, G.Endo: "DNA-DNA Colony-hybridization for viable and quantitative detection of Legionella pneumophila in wastewater" Proceedings of 7th IAWQ Asia-Pacific Conference. (1999)
K.Ishigami、G.Endo:“DNA-DNA 菌落杂交用于废水中嗜肺军团菌的可行和定量检测”第七届 IAWQ 亚太会议记录。
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{{ truncateString('ENDO Ginro', 18)}}的其他基金
Study on utilization technology of oxygen-tolerant denitrifying microorganisms in sewage and wastewater treatment to prevent global warming
耐氧反硝化微生物在污水及废水处理中防治全球变暖的利用技术研究
- 批准号:
21360258 - 财政年份:2009
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of Combinatorial Technology To Simultaneously Accomplish Detection and Purification of the Heavy Metal Contamination.
开发同时完成重金属污染检测与纯化的组合技术。
- 批准号:
16360267 - 财政年份:2004
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research on the new biotechnology for specific detection of environmental contamination by hazardous heavy metals.
环境有害重金属污染特异性检测的新型生物技术研究
- 批准号:
13450216 - 财政年份:2001
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of breeding technology of microorganisms for environmantal purification using natural propagation of genotypes.
开发利用基因型自然繁殖净化环境的微生物育种技术。
- 批准号:
11680580 - 财政年份:1999
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
重金属膜輸送遺伝子とキナーゼ遺伝子の共クローニングによる重金属とリン除去の研究
共克隆重金属膜转运基因与激酶基因去除重金属除磷的研究
- 批准号:
07808063 - 财政年份:1995
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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